The role of advanced oxidation protein products in regulation of dendritic cell function

The basis for this study was the “injury hypothesis,” which holds that release of micro-environmental constituents, such as reactive oxygen species and oxidants, acts as a signal, and potential activator, of dendritic cell (DC)-mediated antigen presentation. Following this oxidative stress, dityrosi...

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Bibliographic Details
Published in:Free radical biology & medicine 2002-03, Vol.32 (5), p.377-385
Main Authors: Alderman, Charles J.J, Shah, Sachit, Foreman, John C, Chain, Benjamin M, Katz, David R
Format: Article
Language:English
Subjects:
Advanced oxidation protein products (AOPP)
Animals
Antigens, CD - immunology
Antigens, CD - metabolism
Cattle
Cell Differentiation
Cell Division
Cells, Cultured
Danger hypothesis
Dendritic cell(s)
Dendritic Cells - physiology
Dityrosine
Free radicals
Granulocyte-Macrophage Colony-Stimulating Factor - metabolism
HLA-DR Antigens - immunology
HLA-DR Antigens - metabolism
Humans
Hypochlorous Acid - metabolism
Immunomagnetic Separation
In Vitro Techniques
Interleukin-4 - metabolism
Lipopolysaccharides - metabolism
Monocytes - immunology
Monocytes - metabolism
Oxidation-Reduction
Oxidative mitogenesis
Oxidative stress
Reactive oxygen species
Reactive Oxygen Species - metabolism
Reactive Oxygen Species - pharmacology
Serum Albumin, Bovine - metabolism
T-Lymphocytes - immunology
T-Lymphocytes - metabolism
Tyrosine - analogs & derivatives
Tyrosine - metabolism
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Summary:The basis for this study was the “injury hypothesis,” which holds that release of micro-environmental constituents, such as reactive oxygen species and oxidants, acts as a signal, and potential activator, of dendritic cell (DC)-mediated antigen presentation. Following this oxidative stress, dityrosine containing cross-linked proteins, advanced oxidation protein products (AOPP), are known to be generated, and we proposed that they may serve as moieties that mediate such signals. Therefore, the effect of AOPP on DCs has been examined in vitro. There were no AOPP-induced changes in DC phenotype as judged by expression of typical surface costimulatory molecules. However, at higher cell concentrations AOPP-treated DCs were more potent inducers in an oxidative mitogenesis assay than controls. Thus, AOPP may act like superantigens, allowing for bypass of upregulation of costimulation, and, either alone or in synergy with oxidants themselves, serving as amplifiers of DC function.
ISSN:0891-5849
1873-4596
DOI:10.1016/S0891-5849(01)00735-3