The ACT-1 plasmid-encoded AmpC β-lactamase is inducible: detection in a complex β-lactamase background

The purpose of this study was to identify the genetic organization and inducibility of blaACT-1 in a clinical isolate of Klebsiella pneumoniae possessing at least five different β-lactamases. The genetic organization of the blaACT-1/ampR region is identical to those of inducible chromosomal ampC gen...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2002-03, Vol.49 (3), p.557-560
Main Authors: Reisbig, Mark D., Hanson, Nancy D.
Format: Article
Language:English
Subjects:
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Bacterial Proteins
Bacteriology
beta-Lactamases - biosynthesis
beta-Lactamases - genetics
Biological and medical sciences
Cefoxitin - pharmacology
DNA, Bacterial - analysis
Enzyme Induction
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Klebsiella pneumoniae - drug effects
Klebsiella pneumoniae - enzymology
Klebsiella pneumoniae - genetics
Medical sciences
Metabolism. Enzymes
Microbiology
Pharmacology. Drug treatments
Plasmids
Polymerase Chain Reaction
RNA, Bacterial - analysis
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Summary:The purpose of this study was to identify the genetic organization and inducibility of blaACT-1 in a clinical isolate of Klebsiella pneumoniae possessing at least five different β-lactamases. The genetic organization of the blaACT-1/ampR region is identical to those of inducible chromosomal ampC genes. RNA analysis using primer extension demonstrated a five-fold increase in blaACT-1 transcript production on exposure to cefoxitin. These findings are significant because induction was detected in a complicated β-lactamase background. In addition, this report is the first to describe an inducible plasmid-encoded AmpC β-lactamase of Enterobacter cloacae origin.
ISSN:0305-7453
1460-2091
1460-2091
DOI:10.1093/jac/49.3.557