Pleiotropic phenotypes of fission yeast defective in ubiquinone-10 production. A study from the abc1Sp (coq8Sp) mutant

We previously constructed two Schizosaccahromyces pombe ubiquinone‐10 (or Coenzyme Q10) less mutants, which are either defective for decaprenyl diphosphate synthase or p‐hydroxybenzoate polyprenyl diphosphate transferase. To further confirm the roles of ubiquinone in S. pombe, we examined the phenot...

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Bibliographic Details
Published in:BioFactors (Oxford) 2003, Vol.18 (1-4), p.229-235
Main Authors: Saiki, Ryoichi, Ogiyama, Yuki, Kainou, Tomohiro, Nishi, Tomoko, Matsuda, Hideyuki, Kawamukai, Makoto
Format: Article
Language:English
Subjects:
antioxidant
Antioxidants - administration & dosage
Copper - pharmacology
coq8
Cysteine - administration & dosage
fission yeast
Glutathione - administration & dosage
Hydrogen Peroxide - pharmacology
Hydrogen Sulfide - metabolism
Mutation
Oxidative Stress
Phenotype
Schizosaccharomyces - genetics
Schizosaccharomyces - growth & development
Schizosaccharomyces - metabolism
sulfide
Ubiquinone - biosynthesis
Ubiquinone - genetics
ubiquinone-10
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Summary:We previously constructed two Schizosaccahromyces pombe ubiquinone‐10 (or Coenzyme Q10) less mutants, which are either defective for decaprenyl diphosphate synthase or p‐hydroxybenzoate polyprenyl diphosphate transferase. To further confirm the roles of ubiquinone in S. pombe, we examined the phenotype of the abc1Sp (coq8Sp) mutant, which is highly speculated to be defective in ubiquinone biosynthesis. We show here that the abc1Sp defective strain did not produce UQ‐10 and could not grow on minimal medium. The abc1Sp‐deficient strain required supplementation with antioxidants such as cysteine or glutathione to grow on minimal medium. In support of the antioxidant function of ubiquinone, the abc1Sp‐deficient strain is sensitive to H2O2 and Cu2+. In addition, expression of the stress inducible ctt1 gene was much induced in the ubiquinone less mutant than wild type. Interestingly, we also found that the abc1‐deficient strain as well as other ubiquinone less mutants produced a significant amount of H2S, which suggests that oxidation of sulfide by ubiquinone may be an important pathway for sulfur metabolism in S. pombe. Thus, analysis of the phenotypes of S. pombe ubiquinone less mutants clearly demonstrate that ubiquinone has multiple functions in the cell apart from being an integral component of the electron transfer system.
ISSN:0951-6433
1872-8081
DOI:10.1002/biof.5520180225