Circular Viral DNA and Anomalous Junction Sequence in PBMC of HIV-Infected Individuals with No Detectable Plasma HIV RNA

Closed circular (cc) forms of extrachromosomal HIV DNA are detected in patients with high viral loads; however, it is unclear whether these forms remain if virus replication is suppressed to undetectable levels by combination antiretroviral therapy. A nested primer polymerase chain reaction amplific...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2002-01, Vol.292 (1), p.1-5
Main Authors: Cara, A., Vargas, J., Keller, M., Jones, S., Mosoian, A., Gurtman, A., Cohen, A., Parkas, V., Wallach, F., Chusid, E., Gelman, I.H., Klotman, M.E.
Format: Article
Language:English
Subjects:
Base Sequence
DNA, Circular - genetics
DNA, Viral - genetics
HIV Infections - virology
HIV Long Terminal Repeat - genetics
HIV-1 - genetics
HIV-1 - physiology
Human immunodeficiency virus
Humans
Leukocytes, Mononuclear - virology
Molecular Sequence Data
RNA, Viral - blood
Sequence Analysis, DNA
viral load
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Summary:Closed circular (cc) forms of extrachromosomal HIV DNA are detected in patients with high viral loads; however, it is unclear whether these forms remain if virus replication is suppressed to undetectable levels by combination antiretroviral therapy. A nested primer polymerase chain reaction amplification assay was used to detect the presence of ccDNA containing two long terminal repeat sequences (2-LTR) in PBMC of patients with low or undetectable plasma HIV RNA. Fifty percent of patients with plasma RNA levels
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.2001.1243