Effect of apoE/ATP-containing liposomes on hepatic energy state

• Published in: Liver international 2003-10, Vol.23 (5), p.379-385
• Main Authors: Chaïb, S., Charrueau, C., Neveux, N., Nakib, S., Chaumeil, J.-C., Cynober, L., De Bandt, J.-P.
• Format: Article
• Language: English
• Subjects: adenosine triphosphate; Adenosine Triphosphate - pharmacology; Animals; apolipoprotein E; Apolipoproteins E - pharmacology; Biological and medical sciences; Energy Metabolism - drug effects; Energy Metabolism - physiology; Fundamental and applied biological sciences. Psychology; Graft Survival; Guinea Pigs; guinea-pig; liposome; Liposomes; Liver - drug effects; Liver - metabolism; Liver Transplantation; Liver. Bile. Biliary tracts; Male; Perfusion; Vertebrates: digestive system
• ISSN: 1478-3223; 1478-3231
• DOI: 10.1034/j.1478-3231.2003.00863.x

: Background/Aims: ATP‐containing liposomes partially prevent ATP depletion in the cold‐stored liver. As hepatocytes can specifically bind apoE, we investigated whether the addition of apoE to large (200 nm) ATP‐containing liposomes increases their uptake by the liver and further improves hepatic energy stores. Methods: Livers from fasted male Hartley guinea‐pigs (231±3 g) were perfused for 90 min under our standard conditions (Control, n=6) or after a single bolus addition of plain liposomes (Lip, n=6), ATP (5 μmol)‐containing liposomes (ATP‐Lip, n=6) or apoE/ATP‐containing liposomes (0.8 or 8 mg apoE/g phospholipids; apoE1‐Lip and apoE10‐Lip, respectively, n=6 in each group). Liposome uptake and its impact on energy and nitrogen metabolism were studied. Results: At its highest concentration, apoE significantly increased liposome uptake (apoE10‐Lip:−9.17±0.69 vs apoE1‐Lip:−6.18±0.44 vs ATP‐Lip:−6.40±0.88 nmol min−1 g−1; P