p53 blocks RuvAB promoted branch migration and modulates resolution of Holliday junctions by RuvC

The Holliday junction is the central intermediate in homologous recombination. Branch migration of this four-stranded DNA structure is a key step in genetic recombination that affects the extent of genetic information exchanged between two parental DNA molecules. Here, we have constructed synthetic...

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Bibliographic Details
Published in:Journal of molecular biology 2002-03, Vol.316 (5), p.1023-1032
Main Authors: Prabhu, Vidya P, Simons, Amanda M, Iwasaki, Hiroshi, Gai, Dahai, Simmons, Daniel T, Chen, Junghuei
Format: Article
Language:English
Subjects:
Animals
Bacterial Proteins - antagonists & inhibitors
Bacterial Proteins - metabolism
Base Pair Mismatch - genetics
Base Sequence
Binding, Competitive
branch migration
DNA - chemistry
DNA - genetics
DNA - metabolism
DNA Helicases
DNA Repair - genetics
DNA Repair - physiology
DNA-Binding Proteins - antagonists & inhibitors
DNA-Binding Proteins - metabolism
Electrophoretic Mobility Shift Assay
Endodeoxyribonucleases - metabolism
Escherichia coli Proteins - antagonists & inhibitors
Escherichia coli Proteins - metabolism
Holliday junction
Holliday junctions
Mice
Models, Genetic
Nucleic Acid Conformation
p53
Recombination, Genetic - genetics
Recombination, Genetic - physiology
RuvAB
RuvAB protein
RuvC
RuvC protein
Tumor Suppressor Protein p53 - metabolism
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Summary:The Holliday junction is the central intermediate in homologous recombination. Branch migration of this four-stranded DNA structure is a key step in genetic recombination that affects the extent of genetic information exchanged between two parental DNA molecules. Here, we have constructed synthetic Holliday junctions to test the effects of p53 on both spontaneous and RuvAB promoted branch migration as well as the effect on resolution of the junction by RuvC. We demonstrate that p53 blocks branch migration, and that cleavage of the Holliday junction by RuvC is modulated by p53. These findings suggest that p53 can block branch migration promoted by proteins such as RuvAB and modulate the cleavage by Holliday junction resolution proteins such as RuvC. These results suggest that p53 could have similar effects on eukaryotic homologues of RuvABC and thus have a direct role in recombinational DNA repair.
ISSN:0022-2836
1089-8638
DOI:10.1006/jmbi.2001.5408