Cysteine proteinases Fas1 and Fas2 are diagnostic markers for Fasciola hepatica infection in alpacas ( Lama pacos)

Circulating antibody against Fasciola hepatica antigens was determined by enzyme-linked immunosorbent assay (ELISA) and immunoelectrophoresis in alpacas naturally exposed to F. hepatica. Serological assay parameters were established by using sera from eight infected animals and seven controls with n...

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Bibliographic Details
Published in:Veterinary parasitology 2002-04, Vol.105 (1), p.21-32
Main Authors: Neyra, Victor, Chavarry, Elizabeth, Espinoza, Jose R
Format: Article
Language:English
Subjects:
Alpacas
Animals
Antibodies, Helminth - blood
Antibodies, Helminth - immunology
Antigens, Helminth - blood
Antigens, Helminth - immunology
Camelids, New World - blood
Camelids, New World - parasitology
Cysteine Endopeptidases - blood
Cysteine Endopeptidases - immunology
Cysteine proteinases
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
Fasciola hepatica
Fasciola hepatica - enzymology
Fasciola hepatica - immunology
Fascioliasis - diagnosis
Fascioliasis - epidemiology
Fascioliasis - veterinary
Feces - parasitology
Female
Immunodiagnosis
Male
Parasite Egg Count - veterinary
Peru - epidemiology
Sensitivity and Specificity
Seroepidemiologic Studies
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Summary:Circulating antibody against Fasciola hepatica antigens was determined by enzyme-linked immunosorbent assay (ELISA) and immunoelectrophoresis in alpacas naturally exposed to F. hepatica. Serological assay parameters were established by using sera from eight infected animals and seven controls with no record of this parasitic infection. Excretory–secretory (ES-) products, Fas1- and Fas2-ELISA were used to survey 307 alpacas from a F. hepatica endemic area in the Peruvian Andes. Seroprevalence of F. hepatica infection varied from 56.7, 64.8 and 66.8% measured by Fas1-, Fas2- and ES-ELISA, respectively. The sensitivity for ES-ELISA was 95%, corresponding Fas1- and Fas2-ELISA sensitivity values were 90 and 95%. In this population, 7% of animals were positive for F. hepatica eggs in faeces, other parasites detected were Trichuris sp. (40%), Nematodirus sp. (34.6%), Lamanema sp. (12.8%) and Eimeria sp. (11.8%). The results show that F. hepatica infected animals elicit circulating antibodies against ES, Fas1 and Fas2. Fas2-ELISA may be proposed as a sensitive assay for the immunodiagnosis of fasciolosis in alpacas.
ISSN:0304-4017
1873-2550
DOI:10.1016/S0304-4017(02)00002-X