CD1d on antigen‐transporting APC and splenic marginal zone B cells promotes NKT cell‐dependent tolerance

CD1d‐reactive NKT cells are required for the development of antigen‐specific T regulatory (Tr) cells responsible for mediating systemic tolerance induced through an immune privileged site suchas the eye. The aim of this study was to elucidate the cellular source of CD1d needed for NKT cell activatio...

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Bibliographic Details
Published in:European journal of immunology 2002-03, Vol.32 (3), p.848-857
Main Authors: Sonoda, Koh‐Hei, Stein‐Streilein, Joan
Format: Article
Language:English
Subjects:
Adoptive Transfer
Animals
Antigen-Presenting Cells - immunology
Antigens, CD1 - genetics
Antigens, CD1 - immunology
Antigens, CD1d
Ascitic Fluid - cytology
B-Lymphocyte Subsets - immunology
B-Lymphocyte Subsets - transplantation
CD1d
CD1d antigen
Cells, Cultured - immunology
Chimera
Crosses, Genetic
Eye - immunology
Female
Hypersensitivity, Delayed - immunology
Immune Tolerance - immunology
Innate immunity
Killer Cells, Natural - immunology
Lymphocyte Activation
MHC class I‐like molecule
Mice
Mice, Inbred C57BL
Mice, Knockout
NKT cell
Peripheral tolerance
Spleen - cytology
Transforming Growth Factor beta - pharmacology
transforming growth factor-^b2
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Summary:CD1d‐reactive NKT cells are required for the development of antigen‐specific T regulatory (Tr) cells responsible for mediating systemic tolerance induced through an immune privileged site suchas the eye. The aim of this study was to elucidate the cellular source of CD1d needed for NKT cell activation. Transforming growth factor β (TGFβ)‐2‐treated peritoneal exudate cells (PEC) functionally resemble "eye‐derived" antigen‐presenting cells (APC) and contribute to the generation of Tr cells both in vitro and in vivo. However, when TGFβ‐2‐treated PEC were pretreated with CD1d‐specific antibodies or lacked CD1d expression they failed to induce Tr cells. In addition, we show that the subpopulation of marginal zone (MZ) B cells within the spleen is necessary for induction of Tr cells and that they also must express CD1d. The role of MZ B in tolerance is novel and previously unexplored. Thus, CD1d is necessarily expressed on putative eye‐derived APC and splenic MZ B cells for efficient generation of Tr cells in CD1d‐reactive NKT cell‐dependent tolerance.
ISSN:0014-2980
1521-4141
DOI:10.1002/1521-4141(200203)32:3<848::AID-IMMU848>3.0.CO;2-I