Simplified method for producing Paracoccidioides brasiliensis exoantigens for use in immunodiffusion tests

A simplified method to produce Paracoccidioides brasiliensis exoantigens for immunodiffusion testing is proposed. It uses technology accessible for small laboratories with few resources in Latin America, where paracoccidioidomycosis is endemic. This procedure may replace the more complex procedure,...

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Bibliographic Details
Published in:Medical mycology (Oxford) 2003-12, Vol.41 (6), p.539-542
Main Authors: Camargo, Z. P., Berzaghi, R., Amaral, C. C., Silva, S. H. M.
Format: Article
Language:English
Subjects:
Antigens, Fungal - biosynthesis
Antigens, Fungal - immunology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Immunodiffusion
Infectious diseases
Medical sciences
Microbiology
Miscellaneous
Mycological methods and techniques used in mycology
Mycology
Mycoses
Paracoccidioides - immunology
Paracoccidioides - isolation & purification
Paracoccidioides - metabolism
Paracoccidioides brasiliensis
Paracoccidioidomycosis - microbiology
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Summary:A simplified method to produce Paracoccidioides brasiliensis exoantigens for immunodiffusion testing is proposed. It uses technology accessible for small laboratories with few resources in Latin America, where paracoccidioidomycosis is endemic. This procedure may replace the more complex procedure, originally proposed by Camargo et al. in 1988, that is currently commonly used. It is based on the production of exoantigen by P. brasiliensis isolate B339, a good secretor of the characteristic 43 000-Da glycoprotein gp43.
ISSN:1369-3786
1460-2709
DOI:10.1080/13693780310001615358