Cathepsin C, Matrix Metalloproteinases, and their Tissue Inhibitors in Gingiva and Gingival Crevicular Fluid from Periodontitis-affected Patients

Successive active phases observed in periodontal diseases may be explained either by a sudden activation of the pro-forms of tissue-stored degradative enzymes such as metalloproteinases (MMPs) or by an imbalance between metalloproteinases and their tissue inhibitors (TIMPs). To discriminate between...

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Bibliographic Details
Published in:Journal of dental research 2002-03, Vol.81 (3), p.174-178
Main Authors: Soell, M., Elkaim, R., Tenenbaum, H.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Biopsy
Cathepsin C - analysis
Cathepsin C - antagonists & inhibitors
Confidence Intervals
Dentistry
Female
Gingiva - enzymology
Gingiva - pathology
Gingival Crevicular Fluid - enzymology
Humans
Image Processing, Computer-Assisted
Male
Matrix Metalloproteinase 1 - analysis
Matrix Metalloproteinase 2 - analysis
Matrix Metalloproteinase 3 - analysis
Matrix Metalloproteinase 9 - analysis
Matrix Metalloproteinase Inhibitors
Matrix Metalloproteinases - analysis
Middle Aged
Periodontitis - enzymology
Periodontitis - pathology
Protease Inhibitors - analysis
Statistics, Nonparametric
Tissue Inhibitor of Metalloproteinase-1 - analysis
Tissue Inhibitor of Metalloproteinase-2 - analysis
Tissue Inhibitor of Metalloproteinases - analysis
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Summary:Successive active phases observed in periodontal diseases may be explained either by a sudden activation of the pro-forms of tissue-stored degradative enzymes such as metalloproteinases (MMPs) or by an imbalance between metalloproteinases and their tissue inhibitors (TIMPs). To discriminate between these two hypotheses, we quantified the levels, the percentage of active form, and the activities of four metalloproteinases (MMPs -1, -2, -3, and -9), as well as the levels of two tissue inhibitors of metalloproteinases (TIMP-1 and -2) and the activity of cathepsin C in tissue extract supernatants and their corresponding gingival crevicular fluid samples collected from periodontitis-affected and healthy patients. Our results supported evidence that tissue destruction results from an imbalance of metalloproteinases over their tissue inhibitors rather than from a sudden activation of the pro-forms of these enzymes. A significant reduction in the activity of cathepsin C also contributed to the degradative process.
ISSN:0022-0345
1544-0591
DOI:10.1177/0810174