Development of an ELISA for detection of antibodies to avian reovirus in chickens

• Published in: Journal of virological methods 2002-04, Vol.102 (1), p.129-138
• Main Authors: Liu, Hung J., Kuo, Liam C., Hu, Yu C., Liao, Ming H., Lien, Yi Y.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Viral - blood; Antibodies, Viral - immunology; Avian reovirus; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Capsid - genetics; Capsid - immunology; Capsid Proteins; Cercopithecus aethiops; Chickens; Cloning, Molecular; ELISA; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - standards; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Gene Expression; Microbiology; Molecular immunology; Neutralization Tests; Orthoreovirus, Avian - genetics; Orthoreovirus, Avian - immunology; Orthoreovirus, Avian - isolation & purification; Poultry Diseases - blood; Poultry Diseases - immunology; Poultry Diseases - virology; Reoviridae Infections - blood; Reoviridae Infections - immunology; Reoviridae Infections - veterinary; Reoviridae Infections - virology; Reverse Transcriptase Polymerase Chain Reaction; RNA-Binding Proteins; Techniques; Vero Cells; Viral Proteins - genetics; Viral Proteins - immunology
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/S0166-0934(02)00010-1

An enzyme-linked immunosorbent assay (ELISA) using the expressed σC and σB proteins which induce neutralizing antibodies as the coating antigen (σC–σB-ELISA) for the detection of antibodies to avian reovirus in chickens was developed and compared with serum neutralization and conventional ELISA tests. These assays were used to examine the sera from chickens vaccinated experimentally and farm chickens. The correlation rate between serum neutralization and a σC–σB-ELISA was 100% (156/156), and that between serum neutralization and conventional ELISA was 89.1% (139/156). The results revealed that preparation of an ELISA by using σC and σB of ARV as the coating antigen in detecting the field chicken sera in comparison with the conventional ELISA gave a titer more correlated to the serum neutralization test. The σC–σB-ELISA showed a higher correlation with the serum neutralization-positive and -negative sera than that obtained with conventional ELISA. This combination antigen may thus be the best suited for preparing an ELISA for improving the determination of the immune status of chicken flocks or for detection of chicken infections with avian reovirus.