Covalent Immobilization of Single Proteins on Mica for Molecular Recognition Force Microscopy

Tether them! A matrix of proteins that are covalently bound to mica with only one short tether per protein was created in order to fulfil all requirements for molecular recognition force microscopy of single proteins (see picture). This immobilization technique produces a high density of individual...

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Bibliographic Details
Published in:Chemphyschem 2003-12, Vol.4 (12), p.1367-1371
Main Authors: Klein, Dionne C. G., Stroh, Cordula M., Jensenius, Henriette, van Es, Maarten, Kamruzzahan, A. S. M., Stamouli, Amalia, Gruber, Hermann J., Oosterkamp, Tjerk H., Hinterdorfer, Peter
Format: Article
Language:English
Subjects:
Aluminum Silicates
Biological and medical sciences
Biotechnology
Cadherins - chemistry
Fundamental and applied biological sciences. Psychology
Lectins - chemistry
Mannose-Binding Lectin - chemistry
Microscopy, Atomic Force
molecular recognition
Pisum sativum
Proteins - chemistry
receptors
scanning probe microscopy
single-molecule studies
surface chemistry
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Summary:Tether them! A matrix of proteins that are covalently bound to mica with only one short tether per protein was created in order to fulfil all requirements for molecular recognition force microscopy of single proteins (see picture). This immobilization technique produces a high density of individual proteins on a flat surface in liquid with extremely low surface roughness.
ISSN:1439-4235
1439-7641
DOI:10.1002/cphc.200300844