A transient RNA interference assay system using Arabidopsis protoplasts

Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2003-12, Vol.67 (12), p.2674-2677
Main Authors: An, C.I. (Osaka Univ., Suita (Japan)), Sawada, A, Fukusaki, E, Kobayashi, A
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
ARABIDOPSIS THALIANA
Biological and medical sciences
Cells, Cultured
DNA Primers - genetics
double-stranded RNA
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant - genetics
Genes, Reporter - genetics
GENETIC VECTORS
Genetic Vectors - genetics
PLANT BIOTECHNOLOGY
PROTOPLASTS
Protoplasts - metabolism
RNA
RNA Interference
RNA, Double-Stranded - genetics
Transfection
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Summary:Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Introduction of dsRNA into protoplasts led to marked silencing of target transgenes. Our assay system would provide a convenient and efficient way to induce RNAi in protoplasts of the model plant Arabidopsis thaliana.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.67.2674