A transient RNA interference assay system using Arabidopsis protoplasts

Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2003-12, Vol.67 (12), p.2674-2677
Main Authors: An, C.I. (Osaka Univ., Suita (Japan)), Sawada, A, Fukusaki, E, Kobayashi, A
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
ARABIDOPSIS THALIANA
Biological and medical sciences
Cells, Cultured
DNA Primers - genetics
double-stranded RNA
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant - genetics
Genes, Reporter - genetics
GENETIC VECTORS
Genetic Vectors - genetics
PLANT BIOTECHNOLOGY
PROTOPLASTS
Protoplasts - metabolism
RNA
RNA Interference
RNA, Double-Stranded - genetics
Transfection
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cited_by cdi_FETCH-LOGICAL-c500t-61ea757b2f79597d9e1760270bb4f4c58b435e763f9f4b42722a3e996b7f88563
cites cdi_FETCH-LOGICAL-c500t-61ea757b2f79597d9e1760270bb4f4c58b435e763f9f4b42722a3e996b7f88563
container_end_page 2677
container_issue 12
container_start_page 2674
container_title Bioscience, biotechnology, and biochemistry
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creator An, C.I. (Osaka Univ., Suita (Japan))
Sawada, A
Fukusaki, E
Kobayashi, A
description Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Introduction of dsRNA into protoplasts led to marked silencing of target transgenes. Our assay system would provide a convenient and efficient way to induce RNAi in protoplasts of the model plant Arabidopsis thaliana.
doi_str_mv 10.1271/bbb.67.2674
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source Oxford Journals Online; EZB Electronic Journals Library
subjects Arabidopsis - genetics
ARABIDOPSIS THALIANA
Biological and medical sciences
Cells, Cultured
DNA Primers - genetics
double-stranded RNA
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant - genetics
Genes, Reporter - genetics
GENETIC VECTORS
Genetic Vectors - genetics
PLANT BIOTECHNOLOGY
PROTOPLASTS
Protoplasts - metabolism
RNA
RNA Interference
RNA, Double-Stranded - genetics
Transfection
title A transient RNA interference assay system using Arabidopsis protoplasts
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