Monoclonal antibody specific to urochordate Botryllus schlosseri pyloric gland

We describe here the development of a new hybridoma cell line, CF12F6, that produces a specific antibody to Botryllus schlosseri (a colonial tunicate). The monoclonal antibody was isotyped as IgG1 (by enzyme-linked immunosorbent assay), and the cellular localization of the antigenic epitope that rea...

Full description

Saved in:
Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 2003-07, Vol.5 (4), p.388-394
Main Authors: Lapidot, Ziva, Paz, Guy, Rinkevich, Baruch
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Ascidiacea
Botryllus schlosseri
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Gastric Mucosa - immunology
Hybridomas
Immunoglobulin G - immunology
Immunohistochemistry
Marine
Urochordata - immunology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We describe here the development of a new hybridoma cell line, CF12F6, that produces a specific antibody to Botryllus schlosseri (a colonial tunicate). The monoclonal antibody was isotyped as IgG1 (by enzyme-linked immunosorbent assay), and the cellular localization of the antigenic epitope that reacts specifically with CF12F6 was confined to the cells of the pyloric gland of the zooid (by immunohistochemistry). The pyloric gland participates in osmoregulation, digestion, glycogen storage, and various other secretion functions that will be studied further by the use of monoclonal antibody CF12F6, the first in botryllid ascidians that recognizes cells of a whole, single organ.
ISSN:1436-2228
1436-2236
DOI:10.1007/s10126-002-0087-9