Identification of rice (Oryza sativa) proteins linked to the cyclin-mediated regulation of the cell cycle

Yeast two-hybrid assays were used to identify rice proteins interacting with two rice cyclins and other proteins potentially involved in cell cycling. The DNA sequences encoding 119 protein fragments identified were then compared by BLAST against proteins in GenBank. The proteins found include myosi...

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Bibliographic Details
Published in:Plant molecular biology 2003-10, Vol.53 (3), p.273-279
Main Authors: Cooper, Bret, Hutchison, Don, Park, Sylvia, Guimil, Sonia, Luginbühl, Peter, Ellero, Cinzia, Goff, Stephen A, Glazebrook, Jane
Format: Article
Language:English
Subjects:
Cell Cycle - genetics
Cell Cycle - physiology
Cyclins - genetics
Cyclins - metabolism
DNA, Complementary - chemistry
DNA, Complementary - genetics
kinesin
Molecular Sequence Data
Oryza - genetics
Oryza sativa
Plant growth
Plant Proteins - genetics
Plant Proteins - metabolism
Protein Binding
Proteins
Rice
Saccharomyces cerevisiae - genetics
Sequence Analysis, DNA
Two-Hybrid System Techniques
Yeasts
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Summary:Yeast two-hybrid assays were used to identify rice proteins interacting with two rice cyclins and other proteins potentially involved in cell cycling. The DNA sequences encoding 119 protein fragments identified were then compared by BLAST against proteins in GenBank. The proteins found include myosin-like proteins, transcription factors, kinesins, centromere proteins and undefined proteins. Based on interactions with cyclins and other elements required for cycling, we believe the undefined proteins may be involved in associated cycling processes. The identification of proteins involved in cell cycle regulation in rice may allow for the control of agronomic traits involving plant growth or development.
ISSN:0167-4412
1573-5028
DOI:10.1023/B:PLAN.0000007001.30865.0f