Analysis of ribosomal RNA intergenic spacer (IGS) sequences in species and populations of Gyrodactylus (Platyhelminthes: Monogenea) from salmonid fish in northern Europe

The intergenic spacer (IGS) region of ribosomal RNA genes was amplified and sequenced from a variety of Gyrodactylus specimens collected from wild and farmed Atlantic salmon Salmo salar, rainbow trout Oncorhynchus mykiss, and grayling Thymallus thymallus, from various locations in Northern Europe. P...

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Bibliographic Details
Published in:Diseases of aquatic organisms 2003-12, Vol.57 (3), p.237-246
Main Authors: CUNNINGHAM, C. O, COLLINS, C. M, MALMBERG, G, MO, T. A
Format: Article
Language:English
Subjects:
Animal helminthic diseases
Animals
Base Sequence
Biological and medical sciences
Cluster Analysis
DNA Primers
DNA, Intergenic - genetics
Europe
Fundamental and applied biological sciences. Psychology
Genetic Variation
Genetics of eukaryotes. Biological and molecular evolution
Gyrodactylus
Gyrodactylus salaris
Gyrodactylus thymalli
Helminthic diseases
Infectious diseases
Invertebrata
Likelihood Functions
Marine
Medical sciences
Models, Genetic
Molecular Sequence Data
Oncorhynchus mykiss
Parasitic diseases
Phylogeny
Platyhelminthes
Platyhelminths - genetics
Population genetics, reproduction patterns
Repetitive Sequences, Nucleic Acid - genetics
Rivers
RNA, Ribosomal - genetics
Salmo salar
Salmonidae
Salmonidae - parasitology
Sequence Analysis, DNA
Species Specificity
Thymallus thymallus
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Summary:The intergenic spacer (IGS) region of ribosomal RNA genes was amplified and sequenced from a variety of Gyrodactylus specimens collected from wild and farmed Atlantic salmon Salmo salar, rainbow trout Oncorhynchus mykiss, and grayling Thymallus thymallus, from various locations in Northern Europe. Phylogenetic analysis of the sequences confirmed the distinction between G. salaris Malmberg, 1957 and G. thymalli Zitnan, 1960, supporting their validity as separate species. G. salaris adapted to rainbow trout are also distinct from the parasites found on Atlantic salmon, supporting the existence of a rainbow-trout form that was initially identified on the basis of morphological differences. Analysis of the IGS did not provide good resolution of different populations of G. salaris sensu stricto, but was consistent with epidemiological evidence which indicates that introduction of the parasite to Norway was recent and limited. The IGS may be helpful in distinguishing forms of G. salaris that are pathogenic to Atlantic salmon from those that are not.
ISSN:0177-5103
1616-1580
DOI:10.3354/dao057237