Detergent-resistant membrane microdomains and apical sorting of GPI-anchored proteins in polarized epithelial cells

Detergent-insoluble microdomains or rafts play a crucial role in many cellular functions: membrane traffic, cell signalling and human diseases. In this work we investigate the role of rafts in the sorting of GPI-anchored proteins in polarized epithelial cells. In contrast to MDCK cells, the majority...

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Bibliographic Details
Published in:International journal of medical microbiology 2002-02, Vol.291 (6-7), p.439-445
Main Authors: PALADINO, Simona, SARNATARO, Daniela, ZURZOLO, Chiara
Format: Article
Language:English
Subjects:
Animals
Bacteriology
Biological and medical sciences
Cell Line
Cell Polarity
Detergents
Epidemiology
Epithelial Cells - metabolism
Fundamental and applied biological sciences. Psychology
Glycosylphosphatidylinositols - metabolism
Membrane Microdomains - chemistry
Membrane Microdomains - metabolism
Membrane Proteins - metabolism
Microbiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Protein Transport
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Summary:Detergent-insoluble microdomains or rafts play a crucial role in many cellular functions: membrane traffic, cell signalling and human diseases. In this work we investigate the role of rafts in the sorting of GPI-anchored proteins in polarized epithelial cells. In contrast to MDCK cells, the majority of endogenous GPI-anchored proteins are sorted to the basolateral surface of Fischer rat thyroid cells (Zurzolo et al., J. Cell Biol. 121, 1031-1039, 1993). We analyzed a set of transfected GPI proteins in order to understand the role of the GPI anchor and of association with rafts for apical sorting. We found that the GPI moiety is necessary but not sufficient for apical sorting of GPI proteins and that the ectodomain has a major role. We propose a new model in which the stabilization of proteins into rafts, probably mediated by interactions between protein ectodomains and a putative receptor, plays a crucial role in apical sorting.
ISSN:1438-4221
1618-0607