Interleukin-1 beta, transforming growth factor beta 1, prostaglandin E2, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients

We analyzed the ability of the bone marrow (BM) stromal cells to achieve confluence and their proliferative capacity in BM primary cultures from 30 untreated lung cancer patients (LCP), 27 breast cancer patients (BCP), and 30 normal controls (NC) when these confluent cells were induced to proliferat...

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Bibliographic Details
Published in:Annals of hematology 2002-02, Vol.81 (2), p.80-85
Main Authors: HONEGGER, A. E, HOFER, E. L, BARANAO, R. I, MACKINLAY, T. A, MACKINLAY, D. A, BULLORSKY, E. O, BORDENAVE, R. H, CHASSEING, N. A
Format: Article
Language:English
Subjects:
Biological and medical sciences
Bone Marrow Cells - metabolism
Bone Marrow Cells - pathology
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Carcinoma, Squamous Cell - metabolism
Carcinoma, Squamous Cell - pathology
Cell Division
Cells, Cultured
Culture Media, Conditioned - analysis
Culture Media, Conditioned - metabolism
Dinoprostone - secretion
Female
Fibroblasts - metabolism
Fibroblasts - pathology
Fibronectins - secretion
General aspects
Humans
Interleukin-1 - secretion
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
Medical sciences
Transforming Growth Factor beta - secretion
Tumors
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Summary:We analyzed the ability of the bone marrow (BM) stromal cells to achieve confluence and their proliferative capacity in BM primary cultures from 30 untreated lung cancer patients (LCP), 27 breast cancer patients (BCP), and 30 normal controls (NC) when these confluent cells were induced to proliferate following four continuous subcultures. Moreover, we evaluated the production of interleukin-1 beta (IL-1beta), transforming growth factor beta 1 (TGF-beta1), fibronectin, and prostaglandin E2 (PGE2) by pure fibroblasts (fourth passage). A fibroblast colony-forming units (CFU-F) assay was used to investigate the proliferative and confluence capacity. Levels of IL-1beta, TGF-beta1, and fibronectin in conditioned mediums (CM) of fibroblast cultures were measured by enzyme-linked immunosorbent assay (ELISA) kit and PGE(2) by radioimmunoassay (RIA) kit. Confluence was achieved in the 60% of LCP and 78% of BCP primary cultures compared with 100% of NC, and only fibroblasts from seven LCP and six BCP cultures had the capacity to proliferate following four subcultures. Levels of IL-1beta were below 10 pg/ml in both patient groups, while NC had a mean value of 5882.57+/-221.61 pg/ml. Levels of TGF-beta1 in BCP were lower than NC values ( P
ISSN:0939-5555
1432-0584
DOI:10.1007/s00277-001-0410-y