Simultaneous analysis of γ-hydroxybutyric acid, γ-butyrolactone, and 1,4-butanediol by micellar electrokinetic chromatography

A micellar electrokinetic chromatography (MEKC) method was optimised for simultaneous analysis of γ-hydroxybutyric acid (GHB), γ-butyrolactone (GBL), and 1,4-butanediol (BD). Best conditions for separation and baseline stability were achieved using a carrier electrolyte comprising 30.0 mM sodium bar...

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Bibliographic Details
Published in:Forensic science international 2002-02, Vol.125 (2), p.113-119
Main Authors: Dahlén, Johan, Vriesman, Thyrsa
Format: Article
Language:English
Subjects:
1,4-Butanediol
4-Butyrolactone - analysis
Biological and medical sciences
Butanediol
Butylene Glycols - analysis
Butyrolactone
Calibration
Capillary electrophoresis
Chromatography
Chromatography, Micellar Electrokinetic Capillary - methods
Correlation coefficient
Correlation coefficients
Drug addictions
Drugs
Electrokinetics
Electrolytes
General aspects. Methods
Hydroxybutyrates - analysis
Medical sciences
Micellar electrokinetic chromatography
Sodium
Sodium dodecyl sulfate
Sodium lauryl sulfate
Solvents
Solvents - analysis
Target detection
Toxicology
γ-Butyrolactone
γ-Hydroxybutyric acid
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Summary:A micellar electrokinetic chromatography (MEKC) method was optimised for simultaneous analysis of γ-hydroxybutyric acid (GHB), γ-butyrolactone (GBL), and 1,4-butanediol (BD). Best conditions for separation and baseline stability were achieved using a carrier electrolyte comprising 30.0 mM sodium barbital and 150.0 mM sodium dodecyl sulphate (SDS) at pH 10.2. Calibration functions were linear, giving correlation coefficients ( r 2) >0.998 for the three target compounds. Limits of detection (LOD) defined as three times the noise, were 5.1 mg/l, 0.34 and 0.25 g/l for GHB, GBL and BD, respectively. The repeatability of migration times and peak areas, expressed as the R.S.D. ( n=9) was better than 0.41 and 3.05%, respectively. Some casework samples were analysed using the optimised conditions.
ISSN:0379-0738
1872-6283
DOI:10.1016/S0379-0738(02)00006-3