Simultaneous analysis of γ-hydroxybutyric acid, γ-butyrolactone, and 1,4-butanediol by micellar electrokinetic chromatography

• Published in: Forensic science international 2002-02, Vol.125 (2), p.113-119
• Main Authors: Dahlén, Johan, Vriesman, Thyrsa
• Format: Article
• Language: English
• Subjects: 1,4-Butanediol; 4-Butyrolactone - analysis; Biological and medical sciences; Butanediol; Butylene Glycols - analysis; Butyrolactone; Calibration; Capillary electrophoresis; Chromatography; Chromatography, Micellar Electrokinetic Capillary - methods; Correlation coefficient; Correlation coefficients; Drug addictions; Drugs; Electrokinetics; Electrolytes; General aspects. Methods; Hydroxybutyrates - analysis; Medical sciences; Micellar electrokinetic chromatography; Sodium; Sodium dodecyl sulfate; Sodium lauryl sulfate; Solvents; Solvents - analysis; Target detection; Toxicology; γ-Butyrolactone; γ-Hydroxybutyric acid
• ISSN: 0379-0738; 1872-6283
• DOI: 10.1016/S0379-0738(02)00006-3

A micellar electrokinetic chromatography (MEKC) method was optimised for simultaneous analysis of γ-hydroxybutyric acid (GHB), γ-butyrolactone (GBL), and 1,4-butanediol (BD). Best conditions for separation and baseline stability were achieved using a carrier electrolyte comprising 30.0 mM sodium barbital and 150.0 mM sodium dodecyl sulphate (SDS) at pH 10.2. Calibration functions were linear, giving correlation coefficients ( r 2) >0.998 for the three target compounds. Limits of detection (LOD) defined as three times the noise, were 5.1 mg/l, 0.34 and 0.25 g/l for GHB, GBL and BD, respectively. The repeatability of migration times and peak areas, expressed as the R.S.D. ( n=9) was better than 0.41 and 3.05%, respectively. Some casework samples were analysed using the optimised conditions.