Immobilization of glucose oxidase in polypyrrole/polytetrahydrofuran graft copolymers

Glucose oxidase (GOD) was immobilized in four different conducting polymer matrices, namely: polypyrrole, (PPy), poly(pyrrole-graft-polytetrahydrofuran), ( 1) and ( 3); and poly(pyrrole-graft-polystyrene/polytetrahydrofuran), ( 2). The kinetic parameters V max and K m, and the optimum temperature we...

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Bibliographic Details
Published in:International journal of biological macromolecules 2002-04, Vol.30 (2), p.81-87
Main Authors: Tirkeş, S, Toppare, L, Alkan, S, Bakir, U, Önen, A, Yağcı, Y
Format: Article
Language:English
Subjects:
Conductive polymers
Electrolysis
Electrolytes
Furans - chemistry
Glucose oxidase
Glucose Oxidase - chemistry
Immobilization
Kinetics
Polymers - chemistry
Polystyrenes - chemistry
Pyrroles - chemistry
Temperature
Time Factors
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Summary:Glucose oxidase (GOD) was immobilized in four different conducting polymer matrices, namely: polypyrrole, (PPy), poly(pyrrole-graft-polytetrahydrofuran), ( 1) and ( 3); and poly(pyrrole-graft-polystyrene/polytetrahydrofuran), ( 2). The kinetic parameters V max and K m, and the optimum temperature were determined for both immobilized and native enzymes. The effect of electrolysis time and several supporting electrolytes, p-toluenesulfonic acid, p-toluene sulfonic acid (PTSA), sodium p-toluene sulfonate, sodium p-toluene sulfonate (NaPTS), and sodium dodecyl sulfate, sodium dodecyl sulfate (SDS), on enzyme immobilization were investigated. The high K m value (59.9 mM) of enzyme immobilized in PPy was decreased via immobilization in graft copolymer matrices of pyrrole. V max, which was 2.25 mM/min for pure PPy, was found as 4.71 mM/min for compound ( 3).
ISSN:0141-8130
1879-0003
DOI:10.1016/S0141-8130(02)00011-9