Lower Limb Ischemia‐Reperfusion Injury Triggers a Systemic Inflammatory Response and Multiple Organ Dysfunction

Restoration of blood flow to an acutely ischemic lower limb may, paradoxically, result in systemic complications and unexpected mortality. We investigated the effect of acute ischemia-perfusion of the lower limb on cytokine production and end organ function. Plasma concentrations of tumor necrosis f...

Full description

Saved in:
Bibliographic Details
Published in:World journal of surgery 2002-01, Vol.26 (1), p.115-121
Main Authors: Yassin, Magdi M.I., Harkin, Denis W., Barros D’Sa, Aires A.B., Halliday, M. Isla, Rowlands, Brian J.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blood and lymphatic vessels
Cardiology. Vascular system
Disease Models, Animal
Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous
Hindlimb - blood supply
Hindlimb - immunology
Interleukin-6 - blood
Kidney - immunology
Kidney - pathology
Liver - immunology
Liver - pathology
Lung - immunology
Lung - pathology
Male
Medical sciences
Multiple Organ Failure - blood
Multiple Organ Failure - etiology
Multiple Organ Failure - immunology
Rats
Rats, Wistar
Reperfusion Injury - blood
Reperfusion Injury - complications
Reperfusion Injury - immunology
Systemic Inflammatory Response Syndrome - blood
Systemic Inflammatory Response Syndrome - etiology
Systemic Inflammatory Response Syndrome - immunology
Time Factors
Tumor Necrosis Factor-alpha - analysis
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Restoration of blood flow to an acutely ischemic lower limb may, paradoxically, result in systemic complications and unexpected mortality. We investigated the effect of acute ischemia-perfusion of the lower limb on cytokine production and end organ function. Plasma concentrations of tumor necrosis factor-alpha (TNF-a) and interleukin-6 (IL-6) were determined in five groups of male Wistar rats: control, 3 hours of bilateral hind limb ischemia alone, and 3 hours of bilateral hind limb ischemia followed by 1 hour, 2 hours, or 3 hours of reperfusion, respectively. In a second experiment, the effect of lower limb ischemia-reperfusion on remote organs (lung, liver, and kidney) was assessed biochemically and histologically. There was a significant increase in plasma concentrations of TNF-a in plasma of animals subjected to 3 hours of bilateral hind limb ischemia followed by 1 hour of reperfusion, 40.1 +/- 4.4 pg/ml, when compared with controls, 22.6 +/- 4.4 pg/ml, or animals in the ischemia-alone group, 16.3 +/- 5.2 (p
ISSN:0364-2313
1432-2323
DOI:10.1007/s00268-001-0169-2