Hypericum perforatum—Chemical profiling and quantitative results of St. John's Wort products by an improved high-performance liquid chromatography method

The analysis of flavonoids, naphthodianthrones, and the phloroglucinol derivative hyperforin in H. perforatum is described in this article. In a 35-min HPLC run nine major compounds could be identified and baseline separated in the methanolic plant extracts. For an optimum separation the mobile phas...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 2002-03, Vol.91 (3), p.623-630
Main Authors: Ganzera, M., Zhao, J., Khan, I.A.
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Calibration
Chromatography, High Pressure Liquid
flavonoids
General pharmacology
HPLC
hyperforin
Hypericum - chemistry
Hypericum perforatum
Mass Spectrometry
Medical sciences
naphthodianthrones
Pharmacology. Drug treatments
Reproducibility of Results
Solvents
Spectrophotometry, Ultraviolet
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Summary:The analysis of flavonoids, naphthodianthrones, and the phloroglucinol derivative hyperforin in H. perforatum is described in this article. In a 35-min HPLC run nine major compounds could be identified and baseline separated in the methanolic plant extracts. For an optimum separation the mobile phase consisted of 10mM ammonium acetate buffer (pH 5.0) and an acetonitrile/methanol mixture; a Synergi MAX-RP 80 Å column (C-12 material) was used as stationary phase. Detection was performed at 270nm, and the identity of the compounds was confirmed in an LC-MS experiment. Commercial St. John's Wort products were analyzed and qualitative and quantitative results are discussed. © 2002 Wiley-Liss, Inc. and the American Pharmaceutical Association J Pharm Sci 91:623–630, 2002
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.10057