Rapid monitoring of recombinant protein products: a comparison of current technologies

Specific measurement of recombinant protein titer in a complex environment during industrial bioprocessing has traditionally relied on labor-intensive and time-consuming immunoassays. In recent years, however, developments in analytical technology have resulted in improved methods for protein produc...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 2002-04, Vol.20 (4), p.149-156
Main Authors: Baker, Kym N, Rendall, Mark H, Patel, Ashvin, Boyd, Paul, Hoare, Mike, Freedman, Robert B, James, David C
Format: Article
Language:English
Subjects:
Automation
Biological and medical sciences
Biosensing Techniques - economics
Biosensing Techniques - methods
Biosensing Techniques - trends
Biosensors
Biotechnology
Capital costs
Chromatography
electrochemiluminescence
Electrochemistry - economics
Electrochemistry - instrumentation
Electrochemistry - trends
ELISA
Enzyme-Linked Immunosorbent Assay - economics
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - trends
Fundamental and applied biological sciences. Psychology
Humans
Immunoassays
Ligands
Luminescent Measurements
Methods. Procedures. Technologies
Molecular Probe Techniques
nephelometry
Nephelometry and Turbidimetry - economics
Nephelometry and Turbidimetry - methods
Nephelometry and Turbidimetry - trends
optical biosensor
Optics and Photonics - instrumentation
Others
peak-rate
Pregnancy
Proteins
recombinant
Recombinant Proteins - analysis
rhIgG1
Technological change
TIMP-1
Various methods and equipments
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Summary:Specific measurement of recombinant protein titer in a complex environment during industrial bioprocessing has traditionally relied on labor-intensive and time-consuming immunoassays. In recent years, however, developments in analytical technology have resulted in improved methods for protein product monitoring during bioprocessing. The choice of product-monitoring technology for a particular bioprocess will depend on a variety of assay factors and instrument-specific factors. In this article, we have compiled an overview of the advantages and disadvantages of the most commonly used technologies used: electrochemiluminescence, optical biosensors, rapid chromatography and nephelometry. The advantages of each technology for measuring both small and large recombinant therapeutic proteins are compared with a conventional enzyme-linked immunosorbent assay (ELISA) technique. Recently, developments in analytical technology have resulted in improved methods for protein product monitoring during bioprocessing. The advantages and disadvantages of different technologies are discussed here.
ISSN:0167-7799
1879-3096
DOI:10.1016/S0167-7799(01)01914-X