Adenovirus infection targets the cellular protein kinase CK2 and RNA-activated protein kinase (PKR) into viral inclusions of the cell nucleus

The effects of the adenovirus infection on the distribution of the cellular protein kinase CK2 and double‐stranded RNA‐activated protein kinase (PKR) were examined at the ultrastructural level. Immunogold labeling revealed the redistribution of CK2 subunits and PKR to morphologically distinct struct...

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Bibliographic Details
Published in:Microscopy research and technique 2002-03, Vol.56 (6), p.465-478
Main Authors: Souquere-Besse, Sylvie, Pichard, Evelyne, Filhol, Odile, Legrand, Valerie, Rosa-Calatrava, Manuel, Hovanessian, Ara G., Cochet, Claude, Puvion-Dutilleul, Francine
Format: Article
Language:English
Subjects:
Adenovirus
adenovirus type 5 infection
Adenoviruses, Human - genetics
Adenoviruses, Human - metabolism
Adenoviruses, Human - pathogenicity
Casein Kinase II
Cell Nucleus - ultrastructure
CK2
clusters of interchromatin granules
compact ring
eIF-2 Kinase - metabolism
HeLa Cells - ultrastructure
HeLa Cells - virology
Humans
In Situ Hybridization
Inclusion Bodies, Viral - enzymology
Microscopy, Electron
pIX bodies
PKR
protein crystal
Protein-Serine-Threonine Kinases - metabolism
RNA, Double-Stranded - metabolism
RNA, Ribosomal - metabolism
RNA, Viral - metabolism
VA1 RNA
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Summary:The effects of the adenovirus infection on the distribution of the cellular protein kinase CK2 and double‐stranded RNA‐activated protein kinase (PKR) were examined at the ultrastructural level. Immunogold labeling revealed the redistribution of CK2 subunits and PKR to morphologically distinct structures of the cell nucleus. The electron‐clear amorphous structures, designated pIX nuclear bodies in our previous work (Rosa‐Calatrava et al., 2001), contained CK2 α and PKR. The protein crystals, which result from the regular assembly of hexon, penton base, and fiber proteins [Boulanger et al. (1970) J Gen Virol 6:329–332], contained CK2 β and PKR. Both viral structures were devoid of viral RNA, including the PKR‐inhibitor VA1 RNA generated by the RNA polymerase III. Instead, VA1 RNA accumulated in PKR‐free viral compact rings in which the viral RNA generated by the RNA polymerase II was excluded. Microsc. Res. Tech. 56:465–478, 2002. © 2002 Wiley‐Liss, Inc.
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.10060