Crystallization and preliminary X-ray characterization of a thermostable pectate lyase from Thermotoga maritima

Pectate lyase is an enzyme involved in the degradation of the pectate portion of the primary plant cell wall. A recombinant pectate lyase from Thermotoga maritima where three of the four cysteine residues have been mutated (C132I, C156N, C194L) has been crystallized. Crystals of the same morphology...

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Published in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2002-04, Vol.58 (4), p.709-711
Main Authors: McDonough, Michael A., Ryttersgaard, Carsten, Bjørnvad, Mads Eskelund, Lo Leggio, Leila, Schülein, Martin, Schrøder Glad, Sanne O., Larsen, Sine
Format: Article
Language:English
Subjects:
Crystallization
Crystallography, X-Ray
Enzyme Stability
Heating
Mutation
pectate lyase
Polysaccharide-Lyases - chemistry
Polysaccharide-Lyases - genetics
Thermotoga maritima - chemistry
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container_issue 4
container_start_page 709
container_title Acta crystallographica. Section D, Biological crystallography.
container_volume 58
creator McDonough, Michael A.
Ryttersgaard, Carsten
Bjørnvad, Mads Eskelund
Lo Leggio, Leila
Schülein, Martin
Schrøder Glad, Sanne O.
Larsen, Sine
description Pectate lyase is an enzyme involved in the degradation of the pectate portion of the primary plant cell wall. A recombinant pectate lyase from Thermotoga maritima where three of the four cysteine residues have been mutated (C132I, C156N, C194L) has been crystallized. Crystals of the same morphology and trigonal space group R3 with similar unit‐cell parameters were obtained under two different conditions. The first, 0.3 M (NH4)H2PO4 pH 4.2, gave crystals with a maximum size of 0.4 × 0.2 × 0.2 mm in one week that diffracted to a resolution of 1.87 Å and had unit‐cell parameters a = b = 80.6, c = 148.8 Å. The second, 0.1 M sodium acetate, 6%(w/v) PEG 4000 pH 6.5, gave the same size crystals in two weeks that diffracted to a resolution of 2.1 Å and had unit‐cell parameters a = b = 80.0, c = 150.1 Å.
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0907-4449
1399-0047
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source Wiley; Alma/SFX Local Collection
subjects Crystallization
Crystallography, X-Ray
Enzyme Stability
Heating
Mutation
pectate lyase
Polysaccharide-Lyases - chemistry
Polysaccharide-Lyases - genetics
Thermotoga maritima - chemistry
title Crystallization and preliminary X-ray characterization of a thermostable pectate lyase from Thermotoga maritima
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