A gene expressed exclusively in acute B lymphoblastic leukemias

Representational difference analysis, a cDNA subtraction approach, was used to identify genes that are expressed in acute leukemia but not in normal hematological tissues. We isolated a cDNA fragment from a cell line derived from a B cell acute lymphoblastic leukemia bearing two Philadelphia chromos...

Full description

Saved in:
Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2004, Vol.83 (1), p.85-94
Main Authors: Vialle-Castellano, Alexandra, Laduron, Sandra, De Plaen, Etienne, Jost, Edgar, Dupont, Sophie, Ameye, Geneviève, Michaux, Lucienne, Coulie, Pierre, Olive, Daniel, Boon, Thierry, van Baren, Nicolas
Format: Article
Language:English
Subjects:
ALL
Alternative Splicing
Biological and medical sciences
BLACE gene
Blotting, Northern
Burkitt Lymphoma - genetics
Burkitt Lymphoma - pathology
cDNA cloning
Cell Line, Tumor
Chromosome Mapping
Chromosomes, Human, Pair 7 - genetics
DNA, Complementary - chemistry
DNA, Complementary - genetics
DNA, Complementary - isolation & purification
Exons
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Genes - genetics
Genes. Genome
Genetics of eukaryotes. Biological and molecular evolution
Hematologic and hematopoietic diseases
Humans
Immunotherapy
In Situ Hybridization, Fluorescence
Introns
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Male
Medical sciences
Middle Aged
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Neoplasm Proteins - genetics
Quantitative RT-PCR
Radiation Hybrid Mapping
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Analysis, DNA
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Representational difference analysis, a cDNA subtraction approach, was used to identify genes that are expressed in acute leukemia but not in normal hematological tissues. We isolated a cDNA fragment from a cell line derived from a B cell acute lymphoblastic leukemia bearing two Philadelphia chromosomes. The cDNA derives from an orphan gene that was named BLACE. BLACE is located in region 7q36 and encodes a major 5.3-kb transcript and several alternatively spliced minor transcripts. Significant expression of BLACE was detected by RT-PCR and quantitative RT-PCR in bone marrow samples from B cell acute lymphoblastic leukemia patients. BLACE was not or was scarcely expressed in other types of hematological malignancies, in normal tissues, and in solid tumors.
ISSN:0888-7543
1089-8646
DOI:10.1016/S0888-7543(03)00209-X