Lipopeptide stimulation of MHC class I-restricted memory cytotoxic T lymphocytes from equine infectious anemia virus-infected horses

• Published in: Vaccine 2002-03, Vol.20 (13), p.1809-1819
• Main Authors: Ridgely, Sherritta L, McGuire, Travis C
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Antigens, Viral - administration & dosage; Antigens, Viral - genetics; Bacteriology; Biological and medical sciences; Cysteine - administration & dosage; Cysteine - analogs & derivatives; Cysteine - immunology; Cytotoxic T lymphocyte epitope; Epitopes - administration & dosage; Epitopes - genetics; Equine infectious anemia; Equine Infectious Anemia - immunology; Equine infectious anemia virus; Fundamental and applied biological sciences. Psychology; Gene Products, env - administration & dosage; Gene Products, env - genetics; Gene Products, env - immunology; Histocompatibility Antigens Class I - metabolism; Horses; Immunologic Memory; In Vitro Techniques; Infectious Anemia Virus, Equine - genetics; Infectious Anemia Virus, Equine - immunology; Lipopeptide; Lipoproteins - genetics; Lipoproteins - immunology; Lipoproteins - pharmacology; Microbiology; Molecular Sequence Data; T-Lymphocytes, Cytotoxic - drug effects; T-Lymphocytes, Cytotoxic - immunology; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Viral Core Proteins - administration & dosage; Viral Core Proteins - genetics; Viral Core Proteins - immunology; Virology
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(01)00517-5

The immunogenicity of equine infectious anemia virus (EIAV) Gag and Env equine leukocyte alloantigen (ELA)-A5.1, -A9, and -A1 restricted cytotoxic T lymphocyte (CTL) epitopes synthesized on multiple antigenic peptide (MAP) system coupled to tripalmitoyl- S-glycerylcysteine (P3C) was evaluated in vitro. P3C–MAP-peptide-stimulated peripheral blood mononuclear cells (PBMCs) from horses, chronically infected with EIAV, had memory CTL (CTLm) similar to that of PBMCs stimulated with either the minimal CTL epitopes, longer peptides containing the CTL epitopes, or EIAV. The stimulated CTL lysed EIAV-infected target cells and the percent specific lysis was dependent on the dose of P3C–MAP-peptide used to stimulate PBMCs in vitro and was peptide specific and ELA-A restricted. The results suggest that these P3C–MAP-peptides can be used as immunogens to stimulate primary immune responses in vivo.