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Lipopeptide stimulation of MHC class I-restricted memory cytotoxic T lymphocytes from equine infectious anemia virus-infected horses
The immunogenicity of equine infectious anemia virus (EIAV) Gag and Env equine leukocyte alloantigen (ELA)-A5.1, -A9, and -A1 restricted cytotoxic T lymphocyte (CTL) epitopes synthesized on multiple antigenic peptide (MAP) system coupled to tripalmitoyl- S-glycerylcysteine (P3C) was evaluated in vit...
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Published in: | Vaccine 2002-03, Vol.20 (13), p.1809-1819 |
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description | The immunogenicity of equine infectious anemia virus (EIAV) Gag and Env equine leukocyte alloantigen (ELA)-A5.1, -A9, and -A1 restricted cytotoxic T lymphocyte (CTL) epitopes synthesized on multiple antigenic peptide (MAP) system coupled to tripalmitoyl-
S-glycerylcysteine (P3C) was evaluated in vitro. P3C–MAP-peptide-stimulated peripheral blood mononuclear cells (PBMCs) from horses, chronically infected with EIAV, had memory CTL (CTLm) similar to that of PBMCs stimulated with either the minimal CTL epitopes, longer peptides containing the CTL epitopes, or EIAV. The stimulated CTL lysed EIAV-infected target cells and the percent specific lysis was dependent on the dose of P3C–MAP-peptide used to stimulate PBMCs in vitro and was peptide specific and ELA-A restricted. The results suggest that these P3C–MAP-peptides can be used as immunogens to stimulate primary immune responses in vivo. |
doi_str_mv | 10.1016/S0264-410X(01)00517-5 |
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S-glycerylcysteine (P3C) was evaluated in vitro. P3C–MAP-peptide-stimulated peripheral blood mononuclear cells (PBMCs) from horses, chronically infected with EIAV, had memory CTL (CTLm) similar to that of PBMCs stimulated with either the minimal CTL epitopes, longer peptides containing the CTL epitopes, or EIAV. The stimulated CTL lysed EIAV-infected target cells and the percent specific lysis was dependent on the dose of P3C–MAP-peptide used to stimulate PBMCs in vitro and was peptide specific and ELA-A restricted. The results suggest that these P3C–MAP-peptides can be used as immunogens to stimulate primary immune responses in vivo.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/S0264-410X(01)00517-5</identifier><identifier>PMID: 11906769</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject><![CDATA[Amino Acid Sequence ; Animals ; Antigens, Viral - administration & dosage ; Antigens, Viral - genetics ; Bacteriology ; Biological and medical sciences ; Cysteine - administration & dosage ; Cysteine - analogs & derivatives ; Cysteine - immunology ; Cytotoxic T lymphocyte epitope ; Epitopes - administration & dosage ; Epitopes - genetics ; Equine infectious anemia ; Equine Infectious Anemia - immunology ; Equine infectious anemia virus ; Fundamental and applied biological sciences. Psychology ; Gene Products, env - administration & dosage ; Gene Products, env - genetics ; Gene Products, env - immunology ; Histocompatibility Antigens Class I - metabolism ; Horses ; Immunologic Memory ; In Vitro Techniques ; Infectious Anemia Virus, Equine - genetics ; Infectious Anemia Virus, Equine - immunology ; Lipopeptide ; Lipoproteins - genetics ; Lipoproteins - immunology ; Lipoproteins - pharmacology ; Microbiology ; Molecular Sequence Data ; T-Lymphocytes, Cytotoxic - drug effects ; T-Lymphocytes, Cytotoxic - immunology ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Viral Core Proteins - administration & dosage ; Viral Core Proteins - genetics ; Viral Core Proteins - immunology ; Virology]]></subject><ispartof>Vaccine, 2002-03, Vol.20 (13), p.1809-1819</ispartof><rights>2002 Elsevier Science Ltd</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-af20f7bbb61898bb59f3a180624a997bedc32aa3c08ccbfa4d1d0c24d99a72a33</citedby><cites>FETCH-LOGICAL-c455t-af20f7bbb61898bb59f3a180624a997bedc32aa3c08ccbfa4d1d0c24d99a72a33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13531883$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11906769$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ridgely, Sherritta L</creatorcontrib><creatorcontrib>McGuire, Travis C</creatorcontrib><title>Lipopeptide stimulation of MHC class I-restricted memory cytotoxic T lymphocytes from equine infectious anemia virus-infected horses</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>The immunogenicity of equine infectious anemia virus (EIAV) Gag and Env equine leukocyte alloantigen (ELA)-A5.1, -A9, and -A1 restricted cytotoxic T lymphocyte (CTL) epitopes synthesized on multiple antigenic peptide (MAP) system coupled to tripalmitoyl-
S-glycerylcysteine (P3C) was evaluated in vitro. P3C–MAP-peptide-stimulated peripheral blood mononuclear cells (PBMCs) from horses, chronically infected with EIAV, had memory CTL (CTLm) similar to that of PBMCs stimulated with either the minimal CTL epitopes, longer peptides containing the CTL epitopes, or EIAV. The stimulated CTL lysed EIAV-infected target cells and the percent specific lysis was dependent on the dose of P3C–MAP-peptide used to stimulate PBMCs in vitro and was peptide specific and ELA-A restricted. The results suggest that these P3C–MAP-peptides can be used as immunogens to stimulate primary immune responses in vivo.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antigens, Viral - administration & dosage</subject><subject>Antigens, Viral - genetics</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cysteine - administration & dosage</subject><subject>Cysteine - analogs & derivatives</subject><subject>Cysteine - immunology</subject><subject>Cytotoxic T lymphocyte epitope</subject><subject>Epitopes - administration & dosage</subject><subject>Epitopes - genetics</subject><subject>Equine infectious anemia</subject><subject>Equine Infectious Anemia - immunology</subject><subject>Equine infectious anemia virus</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Products, env - administration & dosage</subject><subject>Gene Products, env - genetics</subject><subject>Gene Products, env - immunology</subject><subject>Histocompatibility Antigens Class I - metabolism</subject><subject>Horses</subject><subject>Immunologic Memory</subject><subject>In Vitro Techniques</subject><subject>Infectious Anemia Virus, Equine - genetics</subject><subject>Infectious Anemia Virus, Equine - immunology</subject><subject>Lipopeptide</subject><subject>Lipoproteins - genetics</subject><subject>Lipoproteins - immunology</subject><subject>Lipoproteins - pharmacology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>T-Lymphocytes, Cytotoxic - drug effects</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Viral Core Proteins - administration & dosage</subject><subject>Viral Core Proteins - genetics</subject><subject>Viral Core Proteins - immunology</subject><subject>Virology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqFkcFu1DAQhi1ERZfCI4B8oSqHgCeJE_uE0ApopUUcKBI3y7HHqlESp3ZSde998Hq7K3prT5ZG3_yemY-Qd8A-AYPm829WNnVRA_t7xuAjYxzagr8gKxBtVZQcxEuy-o8ck9cp_WOZqkC-IscAkjVtI1fkbuOnMOE0e4s0zX5Yej37MNLg6M_zNTW9ToleFBHTHL2Z0dIBhxC31GznMIdbb-gl7bfDdBVyBRN1MQwUrxc_IvWjQ5PjlkT1iIPX9MbHJRX7es66CjFhekOOnO4Tvj28J-TP92-X6_Ni8-vHxfrrpjA153OhXclc23VdA0KKruPSVRoEa8paS9l2aE1Val0ZJozpnK4tWGbK2kqp21JX1Qk53edOMVwveSM1-GSw7_NweUbVAud124pnQRCV4I2sM3j2NMgZy4EMdijfoyaGlCI6NUU_6LhVwNROqXpQqna-FAP1oFTx3Pf-8MXSDWgfuw4OM_DhAOhkdO-iHo1Pj1yVpQux2_7LnsN84huPUSXjcTRofcw2lA3-mVHuAUnFwN4</recordid><startdate>20020315</startdate><enddate>20020315</enddate><creator>Ridgely, Sherritta L</creator><creator>McGuire, Travis C</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20020315</creationdate><title>Lipopeptide stimulation of MHC class I-restricted memory cytotoxic T lymphocytes from equine infectious anemia virus-infected horses</title><author>Ridgely, Sherritta L ; McGuire, Travis C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-af20f7bbb61898bb59f3a180624a997bedc32aa3c08ccbfa4d1d0c24d99a72a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antigens, Viral - administration & dosage</topic><topic>Antigens, Viral - genetics</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cysteine - administration & dosage</topic><topic>Cysteine - analogs & derivatives</topic><topic>Cysteine - immunology</topic><topic>Cytotoxic T lymphocyte epitope</topic><topic>Epitopes - administration & dosage</topic><topic>Epitopes - genetics</topic><topic>Equine infectious anemia</topic><topic>Equine Infectious Anemia - immunology</topic><topic>Equine infectious anemia virus</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Products, env - administration & dosage</topic><topic>Gene Products, env - genetics</topic><topic>Gene Products, env - immunology</topic><topic>Histocompatibility Antigens Class I - metabolism</topic><topic>Horses</topic><topic>Immunologic Memory</topic><topic>In Vitro Techniques</topic><topic>Infectious Anemia Virus, Equine - genetics</topic><topic>Infectious Anemia Virus, Equine - immunology</topic><topic>Lipopeptide</topic><topic>Lipoproteins - genetics</topic><topic>Lipoproteins - immunology</topic><topic>Lipoproteins - pharmacology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>T-Lymphocytes, Cytotoxic - drug effects</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Viral Core Proteins - administration & dosage</topic><topic>Viral Core Proteins - genetics</topic><topic>Viral Core Proteins - immunology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ridgely, Sherritta L</creatorcontrib><creatorcontrib>McGuire, Travis C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ridgely, Sherritta L</au><au>McGuire, Travis C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lipopeptide stimulation of MHC class I-restricted memory cytotoxic T lymphocytes from equine infectious anemia virus-infected horses</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2002-03-15</date><risdate>2002</risdate><volume>20</volume><issue>13</issue><spage>1809</spage><epage>1819</epage><pages>1809-1819</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>The immunogenicity of equine infectious anemia virus (EIAV) Gag and Env equine leukocyte alloantigen (ELA)-A5.1, -A9, and -A1 restricted cytotoxic T lymphocyte (CTL) epitopes synthesized on multiple antigenic peptide (MAP) system coupled to tripalmitoyl-
S-glycerylcysteine (P3C) was evaluated in vitro. P3C–MAP-peptide-stimulated peripheral blood mononuclear cells (PBMCs) from horses, chronically infected with EIAV, had memory CTL (CTLm) similar to that of PBMCs stimulated with either the minimal CTL epitopes, longer peptides containing the CTL epitopes, or EIAV. The stimulated CTL lysed EIAV-infected target cells and the percent specific lysis was dependent on the dose of P3C–MAP-peptide used to stimulate PBMCs in vitro and was peptide specific and ELA-A restricted. The results suggest that these P3C–MAP-peptides can be used as immunogens to stimulate primary immune responses in vivo.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>11906769</pmid><doi>10.1016/S0264-410X(01)00517-5</doi><tpages>11</tpages></addata></record> |
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subjects | Amino Acid Sequence Animals Antigens, Viral - administration & dosage Antigens, Viral - genetics Bacteriology Biological and medical sciences Cysteine - administration & dosage Cysteine - analogs & derivatives Cysteine - immunology Cytotoxic T lymphocyte epitope Epitopes - administration & dosage Epitopes - genetics Equine infectious anemia Equine Infectious Anemia - immunology Equine infectious anemia virus Fundamental and applied biological sciences. Psychology Gene Products, env - administration & dosage Gene Products, env - genetics Gene Products, env - immunology Histocompatibility Antigens Class I - metabolism Horses Immunologic Memory In Vitro Techniques Infectious Anemia Virus, Equine - genetics Infectious Anemia Virus, Equine - immunology Lipopeptide Lipoproteins - genetics Lipoproteins - immunology Lipoproteins - pharmacology Microbiology Molecular Sequence Data T-Lymphocytes, Cytotoxic - drug effects T-Lymphocytes, Cytotoxic - immunology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Viral Core Proteins - administration & dosage Viral Core Proteins - genetics Viral Core Proteins - immunology Virology |
title | Lipopeptide stimulation of MHC class I-restricted memory cytotoxic T lymphocytes from equine infectious anemia virus-infected horses |
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