Mast cells in Wallerian degeneration: Morphologic and ultrastructural changes

• Published in: Journal of comparative neurology (1911) 2002-04, Vol.445 (3), p.199-210
• Main Authors: Esposito, Barbara, De Santis, Amedeo, Monteforte, Rossella, Baccari, Gabriella Chieffi
• Format: Article
• Language: English
• Subjects: amphibia; Animals; Histology; Mast Cells - pathology; Mast Cells - ultrastructure; Microscopy, Electron; Myelin Sheath - pathology; nerve degeneration; Nerve Regeneration; Peripheral Nerve Injuries; Peripheral Nerves - pathology; Peripheral Nerves - physiopathology; peripheral nervous system; Rana esculenta; Wallerian Degeneration - pathology
• ISSN: 0021-9967; 1096-9861
• DOI: 10.1002/cne.10169

The morphologic and ultrastructural changes of mast cells were followed in degenerating distal and regenerating proximal stumps of frog brachial nerve during Wallerian degeneration. Quantitative analysis included determination of both number and size of mast cells. The mast cell response to injury consisted of an early and a late phase. In the early phase, there was an increase in mast cell numbers in the proximal site of the lesion and a release of Alcian blue material consistent with mediator release. This phase of mast cell activation may be related, through the secretion of biogenic agents such as heparin and histamine, to the increase of endoneurial vessel size and vascular permeability, providing access for macrophages and mast cell precursors. The later phase, which peaked at 40 days after transection in the degenerating distal stump, consisted in the degranulation of the mast cells. These mast cells, closely associated with macrophages and degenerating Schwann cells, released secretory granules into the endoneurial microenvironment. These degranulating mast cells, through the released acid hydrolases, may contribute along with macrophages and Schwann cells, to the degradation of myelin debris. At the same time, mast cells appeared filled with granular content in the regenerating proximal segment. Therefore, we suggest that mast cells in peripheral nerves may play an important role in nerve degenerating and regenerating mechanisms through the secretion of diffusible molecules. J. Comp. Neurol. 445:199–210, 2002. © 2002 Wiley‐Liss, Inc.