Expression of 36-kDa microfibril-associated glycoprotein (MAGP-36) in human keratinocytes and its localization in skin

Microfibril-associated glycoprotein-36 (MAGP-36) is a recently isolated elastin-binding protein and considered to be a member of microfibril-associated glycoproteins (MAGPs). We studied the expression of MAGP-36 in cultured normal human keratinocytes and its localization in the skin. MAGP-36 was fou...

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Bibliographic Details
Published in:Journal of dermatological science 2002, Vol.28 (1), p.60-67
Main Authors: Hirano, Eiichi, Fujimoto, Norihiro, Tajima, Shingo, Akiyama, Minoru, Ishibashi, Akira, Kobayashi, Ryoji, Okamoto, Kouji
Format: Article
Language:English
Subjects:
Aged
Aging - metabolism
Cell Differentiation - physiology
Cells, Cultured
Contractile Proteins - metabolism
Elastin
Glycoproteins
Humans
Keratinocytes - cytology
Keratinocytes - metabolism
Microfibril
Microfibril-associated glycoprotein
Pseudoxanthoma Elasticum - metabolism
Pseudoxanthoma Elasticum - pathology
Reference Values
Skin - metabolism
Skin - radiation effects
Tissue Distribution
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Summary:Microfibril-associated glycoprotein-36 (MAGP-36) is a recently isolated elastin-binding protein and considered to be a member of microfibril-associated glycoproteins (MAGPs). We studied the expression of MAGP-36 in cultured normal human keratinocytes and its localization in the skin. MAGP-36 was found to be expressed in cultured human keratinocytes by Western blot and RT-PCR assays. The levels of MAGP-36 (polypeptide and mRNA) and the number of MAGP-36-producing keratinocytes were greatly increased during Ca 2+-induced differentiation of keratinocytes. Immunohistochemical studies demonstrated that MAGP-36 colocalized with elastic fibers and formed candelabra like-fibers in the superficial dermis of normal skin. In the elderly skin of sun-exposed region, immunoreactivity of MAGP-36 in the superficial dermis disappeared. In the lesional skin of pseudoxanthoma elasticum which is an elastin-related disorder, immunoreactivity of MAGP-36 was found in the accumulation of disintegrated elastic fibers. The results show that MAGP-36 is a component of elastic fibers in the dermis and co-operates with elastin in normal and diseased conditions.
ISSN:0923-1811
1873-569X
DOI:10.1016/S0923-1811(01)00148-7