One-step immunochromatographic assay for screening of coeliac disease

Tissue transglutaminase is the autoantigen that elicits endomysial antibodies, which are the serological hallmarks of coeliac disease. We describe a simple, rapid immunochromatographic assay for IgA and IgG antibodies to transglutaminase, which is highly accurate for diagnosis of this disease. Resul...

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Bibliographic Details
Published in:The Lancet (British edition) 2002-03, Vol.359 (9310), p.945-946
Main Authors: Sorell, Luis, Garrote, Jose A, Acevedo, Boris, Arranz, Eduardo
Format: Article
Language:English
Subjects:
Antibodies
Antibodies, Monoclonal - blood
Antibodies, Monoclonal - isolation & purification
Assaying
Autoimmune diseases
Biological and medical sciences
Biopsy
Bowel disease
Celiac disease
Celiac Disease - diagnosis
Celiac Disease - immunology
Chromatography
Enzyme-Linked Immunosorbent Assay - methods
Gastroenterology. Liver. Pancreas. Abdomen
Gastrointestinal diseases
Gluten
Gold
Humans
Immunoglobulin A
Immunoglobulin A - blood
Immunoglobulin A - immunology
Immunoglobulin G
Immunoglobulin G - blood
Immunoglobulin G - immunology
Immunoglobulins
Immunological methods for diagnosis and exploration
Immunopathology
Medical diagnosis
Medical sciences
Medical screening
Membranes
Other diseases. Semiology
Patients
Pore size
Screening
Serological tests
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Transglutaminase 2
Transglutaminases - blood
Transglutaminases - immunology
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Description
Summary:Tissue transglutaminase is the autoantigen that elicits endomysial antibodies, which are the serological hallmarks of coeliac disease. We describe a simple, rapid immunochromatographic assay for IgA and IgG antibodies to transglutaminase, which is highly accurate for diagnosis of this disease. Results were positive for all samples from 50 untreated coeliac patients, and negative for 40 non-coeliac patients with gastrointestinal disorders. The assay seems to be a useful alternative to biopsy for mass screening for coeliac disease.
ISSN:0140-6736
1474-547X
DOI:10.1016/S0140-6736(02)08046-7