10q25.3 (DMBT1) copy number changes in astrocytoma grades II and IV

In the literature, it has been suggested that loss of the 10q25‐26 region, including the DMBT1 gene (10q25.3), is correlated with initiation and/or malignant progression of astrocytomas, although the results of the studies on the loss of heterozygosity that led to this assumption are not unequivocal...

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Published in:Genes chromosomes & cancer 2004-01, Vol.39 (1), p.22-28
Main Authors: Wessels, Peter H., Twijnstra, Albert, Kubat, Bela, Ummelen, Monique I.J., Claessen, Sandra M.H., Sciot, Raf, Merlo, Adrian, Ramaekers, Frans C.S., Speel, Ernst J.M., Hopman, Anton H.N.
Format: Article
Language:English
Subjects:
Adult
Aged
Agglutinins
Astrocytoma - chemistry
Astrocytoma - genetics
Brain Neoplasms - genetics
Chromosomes, Human, Pair 10 - genetics
Female
Gene Dosage
Glioblastoma - chemistry
Glioblastoma - genetics
Humans
Immunohistochemistry
In Situ Hybridization, Fluorescence
Male
Middle Aged
Receptors, Cell Surface - genetics
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Summary:In the literature, it has been suggested that loss of the 10q25‐26 region, including the DMBT1 gene (10q25.3), is correlated with initiation and/or malignant progression of astrocytomas, although the results of the studies on the loss of heterozygosity that led to this assumption are not unequivocal. For this reason, using double‐target fluorescence in situ hybridization, we compared copy number changes of 10q25.3 to those of the pericentromeric region (10q12) in 10 cases each of astrocytoma grades II and IV. The same specimens were analyzed for copy number changes of chromosome 1, as a marker for polyploidy, and chromosome 7, which is often gained in astrocytomas of all grades. Our results show that selective loss of the 10q25.3 region was present in 2 of 10 specimens in both astrocytoma grade II and grade IV, occurring only in tumors with polysomy for 10q12. Furthermore, astrocytoma grade II often showed polyploidy for chromosomes 1, 7, and 10 (8 of 10 specimens). In addition, astrocytoma grade IV frequently exhibited losses of chromosome 10 in a high percentage of nuclei. Although based on a small number of cases, the results clearly show that loss of the 10q25.3 region is uncommon in astrocytoma grade II and mostly coincident with loss of chromosome 10 in grade IV tumors. These data indicate that selective loss of the 10q25.3 region, including the DMBT1 gene, is not an initiating event in the genesis of astrocytoma grade II. © 2003 Wiley‐Liss, Inc.
ISSN:1045-2257
1098-2264
DOI:10.1002/gcc.10288