Human bone marrow stromal cell responses on electrospun silk fibroin mats

Fibers with nanoscale diameters provide benefits due to high surface area for biomaterial scaffolds. In this study electrospun silk fibroin-based fibers with average diameter 700±50 nm were prepared from aqueous regenerated silkworm silk solutions. Adhesion, spreading and proliferation of human bone...

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Bibliographic Details
Published in:Biomaterials 2004-03, Vol.25 (6), p.1039-1047
Main Authors: Jin, Hyoung-Joon, Chen, Jingsong, Karageorgiou, Vassilis, Altman, Gregory H., Kaplan, David L.
Format: Article
Language:English
Subjects:
Adolescent
Adsorption
Animals
Biocompatible Materials - chemical synthesis
Biocompatible Materials - chemistry
Biomimetic Materials - chemistry
Biomimetic Materials - metabolism
Bombyx - chemistry
Bone Marrow Cells - cytology
Bone Marrow Cells - physiology
Cell Adhesion - physiology
Cell Division - physiology
Cells, Cultured
Culture Techniques - methods
Electrochemistry - methods
Electrospinning
Extracellular Matrix - chemistry
Extracellular Matrix - metabolism
Extracellular Matrix - ultrastructure
Fibroins - chemistry
Fibroins - ultrastructure
Human bone marrow stem cells (BMSCs)
Humans
Insect Proteins - chemistry
Manufactured Materials - analysis
Materials Testing
Membranes, Artificial
Nano-fiber
Scaffolds
Silk
Silkworm silk
Stromal Cells - cytology
Stromal Cells - physiology
Textiles
Tissue Engineering - methods
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Summary:Fibers with nanoscale diameters provide benefits due to high surface area for biomaterial scaffolds. In this study electrospun silk fibroin-based fibers with average diameter 700±50 nm were prepared from aqueous regenerated silkworm silk solutions. Adhesion, spreading and proliferation of human bone marrow stromal cells (BMSCs) on these silk matrices was studied. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the electrospun silk matrices supported BMSC attachment and proliferation over 14 days in culture similar to native silk fibroin (∼15 μm fiber diameter) matrices. The ability of electrospun silk matrices to support BMSC attachment, spreading and growth in vitro, combined with a biocompatibility and biodegradable properties of the silk protein matrix, suggest potential use of these biomaterial matrices as scaffolds for tissue engineering.
ISSN:0142-9612
1878-5905
DOI:10.1016/S0142-9612(03)00609-4