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Detection of von Willebrand factor-cleaving protease (ADAMTS-13) in human platelets
The hemostatic activity of von Willebrand factor (vWF) is strongly dependent on its multimeric structure, with the highest activity in ‘unusually large’ multimers secreted from endothelial cells. The multimeric structure is regulated by a plasma protease, vWF-cleaving protease (vWF-CP, or ADAMTS-13)...
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| Published in: | Biochemical and biophysical research communications 2004-01, Vol.313 (1), p.212-216 |
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| cites | cdi_FETCH-LOGICAL-c420t-e4b08afc71d9821284a9a71907078e76aa6ff42112f130b5e88db50fa4fef7bf3 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Suzuki, Misako Murata, Mitsuru Matsubara, Yumiko Uchida, Toshihiro Ishihara, Hiroaki Shibano, Toshiro Ashida, Shin-ichiro Soejima, Kenji Okada, Yasunori Ikeda, Yasuo |
| description | The hemostatic activity of von Willebrand factor (vWF) is strongly dependent on its multimeric structure, with the highest activity in ‘unusually large’ multimers secreted from endothelial cells. The multimeric structure is regulated by a plasma protease, vWF-cleaving protease (vWF-CP, or ADAMTS-13). ADAMTS-13 mRNA is variably expressed in liver and other tissues. Because 15–25% of circulating vWF is stored in platelets, the presence and function of ADAMTS-13 in platelets are important issues. Here we report ADAMTS-13 expression in human platelets. Western blot analysis and flow cytometric analysis on permeabilized platelets revealed the presence of ADAMTS-13 protein. Real-time PCR demonstrated that ADAMTS-13 mRNA is present in platelets of six healthy volunteers, with little quantitative difference. The presence of ADAMTS-13 in platelets may imply the functional role of this enzyme in the local regulation of platelet function at the site of vascular injury or thrombus formation, and provide a useful tool for the analysis of structure and function of this enzyme. |
| doi_str_mv | 10.1016/j.bbrc.2003.11.111 |
| format | article |
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The presence of ADAMTS-13 in platelets may imply the functional role of this enzyme in the local regulation of platelet function at the site of vascular injury or thrombus formation, and provide a useful tool for the analysis of structure and function of this enzyme.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2003.11.111</identifier><identifier>PMID: 14672719</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Actins - analysis ; Actins - biosynthesis ; ADAM Proteins ; ADAMTS-13 ; ADAMTS13 Protein ; Antigens, CD20 - analysis ; Antigens, CD20 - biosynthesis ; Blood Platelets - enzymology ; Blotting, Western ; Expression ; Flow Cytometry ; Gene Expression ; Humans ; Metalloendopeptidases - biosynthesis ; Metalloendopeptidases - blood ; Platelets ; Polymerase Chain Reaction - methods ; Real-time PCR ; RNA, Messenger - analysis ; RNA, Messenger - biosynthesis ; Thrombosis ; TTP ; von Willebrand Factor - metabolism ; vWF ; vWF-CP</subject><ispartof>Biochemical and biophysical research communications, 2004-01, Vol.313 (1), p.212-216</ispartof><rights>2003 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-e4b08afc71d9821284a9a71907078e76aa6ff42112f130b5e88db50fa4fef7bf3</citedby><cites>FETCH-LOGICAL-c420t-e4b08afc71d9821284a9a71907078e76aa6ff42112f130b5e88db50fa4fef7bf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14672719$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suzuki, Misako</creatorcontrib><creatorcontrib>Murata, Mitsuru</creatorcontrib><creatorcontrib>Matsubara, Yumiko</creatorcontrib><creatorcontrib>Uchida, Toshihiro</creatorcontrib><creatorcontrib>Ishihara, Hiroaki</creatorcontrib><creatorcontrib>Shibano, Toshiro</creatorcontrib><creatorcontrib>Ashida, Shin-ichiro</creatorcontrib><creatorcontrib>Soejima, Kenji</creatorcontrib><creatorcontrib>Okada, Yasunori</creatorcontrib><creatorcontrib>Ikeda, Yasuo</creatorcontrib><title>Detection of von Willebrand factor-cleaving protease (ADAMTS-13) in human platelets</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The hemostatic activity of von Willebrand factor (vWF) is strongly dependent on its multimeric structure, with the highest activity in ‘unusually large’ multimers secreted from endothelial cells. The multimeric structure is regulated by a plasma protease, vWF-cleaving protease (vWF-CP, or ADAMTS-13). ADAMTS-13 mRNA is variably expressed in liver and other tissues. Because 15–25% of circulating vWF is stored in platelets, the presence and function of ADAMTS-13 in platelets are important issues. Here we report ADAMTS-13 expression in human platelets. Western blot analysis and flow cytometric analysis on permeabilized platelets revealed the presence of ADAMTS-13 protein. Real-time PCR demonstrated that ADAMTS-13 mRNA is present in platelets of six healthy volunteers, with little quantitative difference. The presence of ADAMTS-13 in platelets may imply the functional role of this enzyme in the local regulation of platelet function at the site of vascular injury or thrombus formation, and provide a useful tool for the analysis of structure and function of this enzyme.</description><subject>Actins - analysis</subject><subject>Actins - biosynthesis</subject><subject>ADAM Proteins</subject><subject>ADAMTS-13</subject><subject>ADAMTS13 Protein</subject><subject>Antigens, CD20 - analysis</subject><subject>Antigens, CD20 - biosynthesis</subject><subject>Blood Platelets - enzymology</subject><subject>Blotting, Western</subject><subject>Expression</subject><subject>Flow Cytometry</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Metalloendopeptidases - biosynthesis</subject><subject>Metalloendopeptidases - blood</subject><subject>Platelets</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Real-time PCR</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Thrombosis</subject><subject>TTP</subject><subject>von Willebrand Factor - metabolism</subject><subject>vWF</subject><subject>vWF-CP</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNp9kEtLw0AUhQdRbH38AReSlegi9d5pmknATWl9QcVFK7obJpM7OiVN6kxS8N87pQV3woWzOedw7sfYBcIAAdPb5aAonB5wgOEAMRwesD5CDjFHSA5ZHwDSmOf40WMn3i8BEJM0P2a9IIILzPtsPqWWdGubOmpMtAnybquKCqfqMjJKt42LdUVqY-vPaO2alpSn6Ho8Hb8s5jEObyJbR1_dStXRulItVdT6M3ZkVOXpfK-n7O3hfjF5imevj8-T8SzWCYc2pqSATBktsMwzjjxLVK7CKBAgMhKpUqkxCUfkBodQjCjLymIERiWGjCjM8JRd7XrDru-OfCtX1muqKlVT03kpcDQS4dFg5Dujdo33joxcO7tS7kciyC1KuZRblHKLUiKGwxC63Ld3xYrKv8ieXTDc7QwUftxYctJrS7Wm0rqAVJaN_a__F48sg-I</recordid><startdate>20040102</startdate><enddate>20040102</enddate><creator>Suzuki, Misako</creator><creator>Murata, Mitsuru</creator><creator>Matsubara, Yumiko</creator><creator>Uchida, Toshihiro</creator><creator>Ishihara, Hiroaki</creator><creator>Shibano, Toshiro</creator><creator>Ashida, Shin-ichiro</creator><creator>Soejima, Kenji</creator><creator>Okada, Yasunori</creator><creator>Ikeda, Yasuo</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040102</creationdate><title>Detection of von Willebrand factor-cleaving protease (ADAMTS-13) in human platelets</title><author>Suzuki, Misako ; Murata, Mitsuru ; Matsubara, Yumiko ; Uchida, Toshihiro ; Ishihara, Hiroaki ; Shibano, Toshiro ; Ashida, Shin-ichiro ; Soejima, Kenji ; Okada, Yasunori ; Ikeda, Yasuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-e4b08afc71d9821284a9a71907078e76aa6ff42112f130b5e88db50fa4fef7bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Actins - analysis</topic><topic>Actins - biosynthesis</topic><topic>ADAM Proteins</topic><topic>ADAMTS-13</topic><topic>ADAMTS13 Protein</topic><topic>Antigens, CD20 - analysis</topic><topic>Antigens, CD20 - biosynthesis</topic><topic>Blood Platelets - enzymology</topic><topic>Blotting, Western</topic><topic>Expression</topic><topic>Flow Cytometry</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Metalloendopeptidases - biosynthesis</topic><topic>Metalloendopeptidases - blood</topic><topic>Platelets</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Real-time PCR</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Thrombosis</topic><topic>TTP</topic><topic>von Willebrand Factor - metabolism</topic><topic>vWF</topic><topic>vWF-CP</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suzuki, Misako</creatorcontrib><creatorcontrib>Murata, Mitsuru</creatorcontrib><creatorcontrib>Matsubara, Yumiko</creatorcontrib><creatorcontrib>Uchida, Toshihiro</creatorcontrib><creatorcontrib>Ishihara, Hiroaki</creatorcontrib><creatorcontrib>Shibano, Toshiro</creatorcontrib><creatorcontrib>Ashida, Shin-ichiro</creatorcontrib><creatorcontrib>Soejima, Kenji</creatorcontrib><creatorcontrib>Okada, Yasunori</creatorcontrib><creatorcontrib>Ikeda, Yasuo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suzuki, Misako</au><au>Murata, Mitsuru</au><au>Matsubara, Yumiko</au><au>Uchida, Toshihiro</au><au>Ishihara, Hiroaki</au><au>Shibano, Toshiro</au><au>Ashida, Shin-ichiro</au><au>Soejima, Kenji</au><au>Okada, Yasunori</au><au>Ikeda, Yasuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of von Willebrand factor-cleaving protease (ADAMTS-13) in human platelets</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2004-01-02</date><risdate>2004</risdate><volume>313</volume><issue>1</issue><spage>212</spage><epage>216</epage><pages>212-216</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The hemostatic activity of von Willebrand factor (vWF) is strongly dependent on its multimeric structure, with the highest activity in ‘unusually large’ multimers secreted from endothelial cells. The multimeric structure is regulated by a plasma protease, vWF-cleaving protease (vWF-CP, or ADAMTS-13). ADAMTS-13 mRNA is variably expressed in liver and other tissues. Because 15–25% of circulating vWF is stored in platelets, the presence and function of ADAMTS-13 in platelets are important issues. Here we report ADAMTS-13 expression in human platelets. Western blot analysis and flow cytometric analysis on permeabilized platelets revealed the presence of ADAMTS-13 protein. Real-time PCR demonstrated that ADAMTS-13 mRNA is present in platelets of six healthy volunteers, with little quantitative difference. 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| subjects | Actins - analysis Actins - biosynthesis ADAM Proteins ADAMTS-13 ADAMTS13 Protein Antigens, CD20 - analysis Antigens, CD20 - biosynthesis Blood Platelets - enzymology Blotting, Western Expression Flow Cytometry Gene Expression Humans Metalloendopeptidases - biosynthesis Metalloendopeptidases - blood Platelets Polymerase Chain Reaction - methods Real-time PCR RNA, Messenger - analysis RNA, Messenger - biosynthesis Thrombosis TTP von Willebrand Factor - metabolism vWF vWF-CP |
| title | Detection of von Willebrand factor-cleaving protease (ADAMTS-13) in human platelets |
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