Use of bioaffinity interactions in electrokinetically controlled assays on microfabricated devices

• Published in: Electrophoresis 2002-03, Vol.23 (6), p.823-835
• Main Authors: Guijt, Rosanne M., Baltussen, Erik, van Dedem, Gijs W. K.
• Format: Article
• Language: English
• Subjects: Affinity interactions; Binding constants; Electrophoresis; Electrophoresis, Capillary - methods; Humans; Immunoassay - methods; Molecular recognition; Review; ν-Total analysis system
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/1522-2683(200203)23:6<823::AID-ELPS823>3.0.CO;2-Z

In this contribution, the role of bioaffinity interactions on electrokinetically controlled microfabricated devices is reviewed. Interesting applications reported in the literature include enzymatic assays, where enzyme and enzyme inhibition kinetics were studied, often in combination with electrophoretic separation. Attention is paid towards developments that could lead to implementation of electrokinetically controlled microdevices in high‐throughput screening. Furthermore, enzyme‐facilitated detection in combination with electrophoretic separation on microdevices is discussed. Various types of immunoassays have been implemented on the microchip format. The selectivity of antibody‐antigen interaction has been exploited for the detection of analytes in complex sample matrices as required, for example, in clinical chemistry. Binding kinetics as well as stoichiometry were studied in chip‐based assays. Automated mixing protocols as well as the demonstration of a parallel immunoassay allow implementation of microdevices in high‐throughput screening. Furthermore, demonstration of immunoassays on cheap polymeric microdevices opens the way towards the fabrication of disposable devices, a requirement for commercialization and therefore for application in routine analyses.