The effect of laminin and its peptide SIKVAV on a human salivary gland myoepithelioma cell line

We have demonstrated that the basement membrane regulates the myoepithelioma. We are now studying the effect of laminin, a basement membrane protein, in the morphology of a cell line (M1) derived from human salivary gland plasmacytoid myoepithelioma. These cells were grown inside a three-dimensional...

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Bibliographic Details
Published in:Oral oncology 2004, Vol.40 (1), p.36-42
Main Authors: Capuano, A.C.T, Jaeger, R.G
Format: Article
Language:English
Subjects:
Basement membrane
Biological and medical sciences
Cell culture
Cell Line, Tumor
Extracellular matrix
Humans
Immunohistochemistry
Laminin
Laminin - pharmacology
Medical sciences
Microscopy, Electron
Muscle, Smooth - metabolism
Myoepithelioma
Myoepithelioma - pathology
Oligopeptides - pharmacology
Otorhinolaryngology. Stomatology
Salivary gland neoplasms
Salivary Gland Neoplasms - pathology
Tumors
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Summary:We have demonstrated that the basement membrane regulates the myoepithelioma. We are now studying the effect of laminin, a basement membrane protein, in the morphology of a cell line (M1) derived from human salivary gland plasmacytoid myoepithelioma. These cells were grown inside a three-dimensional preparation of laminin-1. Phenotype differences were assessed by light and transmission electron microscopy. In addition, we analysed the effect of a molecular domain of laminin-1, the peptide SIKVAV, on M1 cells. This peptide was chosen because it is effective in cell proliferation and differentiation. M1 cells grown inside laminin-1 were mostly plasmacytoid-like, while cells treated by SIKVAV showed light and electron microscopic features of typical plasmacytoid cells. This peptide also modulated smooth-muscle actin expression in M1 cells. We demonstrated that laminin-1 and its derived peptide SIKVAV morphoregulates myoepithelioma cells in culture.
ISSN:1368-8375
1879-0593
DOI:10.1016/S1368-8375(03)00130-1