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In Vitro Studies of a Photo-oxidized Bovine Articular Cartilage

Bovine articular cartilage was photo‐oxidized and cultured with native articular bovine cartilage and synovial membrane to study the interaction between these tissues mimicking the physiological situation in the joint. The photo‐oxidation was applied as a pretreatment of cartilage for future use in...

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Published in:Journal of veterinary medicine. Series A 2002-02, Vol.49 (1), p.39-45
Main Authors: AKENS, M. K., VON RECHENBERG, B., BITTMANN, P., NADLER, D., ZLINSZKY, K., AUER, J. A.
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cited_by cdi_FETCH-LOGICAL-c4037-e6484ec77bd2530e7aaebf6e29ac555b7a314812332f014ba466dd4400a455bf3
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container_title Journal of veterinary medicine. Series A
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creator AKENS, M. K.
VON RECHENBERG, B.
BITTMANN, P.
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ZLINSZKY, K.
AUER, J. A.
description Bovine articular cartilage was photo‐oxidized and cultured with native articular bovine cartilage and synovial membrane to study the interaction between these tissues mimicking the physiological situation in the joint. The photo‐oxidation was applied as a pretreatment of cartilage for future use in cartilage resurfacing procedures in joints. Properties of the transplant were assessed by testing the production of local mediators, such as nitric oxide (NO) and prostaglandin E2 (PGE2), and neutral metalloproteinase activities under normal conditions and after stimulation with various stimulants representative of inflammatory changes in pathophysiological conditions. Unlike normal cartilage photo‐oxidized cartilage did not release significant amounts of NO and PGE2 and showed less gelatinolytic and caseinolytic activity compared to native bovine articular cartilage. Enzyme activity of the combined cultures was at a level intermediate between that of photo‐oxidized cartilage and native cartilage cultures alone. In contrast to normal cartilage, living chondrocytes were not visible in photo‐oxidized cartilage using live/dead staining. These results indicate, that the photo‐oxidized cartilage may have a beneficial effect on adjacent native host cartilage and therefore be a suitable transplant for use in in vivo experiments.
doi_str_mv 10.1046/j.1439-0442.2002.00387.x
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subjects Animals
Cartilage, Articular - cytology
Cartilage, Articular - metabolism
Cartilage, Articular - radiation effects
Cattle
Cells, Cultured
Dinoprostone - biosynthesis
Humerus
Light
Metalloendopeptidases - biosynthesis
Nitric Oxide - biosynthesis
Oxidation-Reduction
Shoulder Joint
Synovial Membrane - cytology
Synovial Membrane - metabolism
Synovial Membrane - radiation effects
title In Vitro Studies of a Photo-oxidized Bovine Articular Cartilage
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