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In Vitro Studies of a Photo-oxidized Bovine Articular Cartilage
Bovine articular cartilage was photo‐oxidized and cultured with native articular bovine cartilage and synovial membrane to study the interaction between these tissues mimicking the physiological situation in the joint. The photo‐oxidation was applied as a pretreatment of cartilage for future use in...
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Published in: | Journal of veterinary medicine. Series A 2002-02, Vol.49 (1), p.39-45 |
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container_title | Journal of veterinary medicine. Series A |
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creator | AKENS, M. K. VON RECHENBERG, B. BITTMANN, P. NADLER, D. ZLINSZKY, K. AUER, J. A. |
description | Bovine articular cartilage was photo‐oxidized and cultured with native articular bovine cartilage and synovial membrane to study the interaction between these tissues mimicking the physiological situation in the joint. The photo‐oxidation was applied as a pretreatment of cartilage for future use in cartilage resurfacing procedures in joints. Properties of the transplant were assessed by testing the production of local mediators, such as nitric oxide (NO) and prostaglandin E2 (PGE2), and neutral metalloproteinase activities under normal conditions and after stimulation with various stimulants representative of inflammatory changes in pathophysiological conditions. Unlike normal cartilage photo‐oxidized cartilage did not release significant amounts of NO and PGE2 and showed less gelatinolytic and caseinolytic activity compared to native bovine articular cartilage. Enzyme activity of the combined cultures was at a level intermediate between that of photo‐oxidized cartilage and native cartilage cultures alone. In contrast to normal cartilage, living chondrocytes were not visible in photo‐oxidized cartilage using live/dead staining. These results indicate, that the photo‐oxidized cartilage may have a beneficial effect on adjacent native host cartilage and therefore be a suitable transplant for use in in vivo experiments. |
doi_str_mv | 10.1046/j.1439-0442.2002.00387.x |
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K. ; VON RECHENBERG, B. ; BITTMANN, P. ; NADLER, D. ; ZLINSZKY, K. ; AUER, J. A.</creator><creatorcontrib>AKENS, M. K. ; VON RECHENBERG, B. ; BITTMANN, P. ; NADLER, D. ; ZLINSZKY, K. ; AUER, J. A.</creatorcontrib><description>Bovine articular cartilage was photo‐oxidized and cultured with native articular bovine cartilage and synovial membrane to study the interaction between these tissues mimicking the physiological situation in the joint. The photo‐oxidation was applied as a pretreatment of cartilage for future use in cartilage resurfacing procedures in joints. Properties of the transplant were assessed by testing the production of local mediators, such as nitric oxide (NO) and prostaglandin E2 (PGE2), and neutral metalloproteinase activities under normal conditions and after stimulation with various stimulants representative of inflammatory changes in pathophysiological conditions. Unlike normal cartilage photo‐oxidized cartilage did not release significant amounts of NO and PGE2 and showed less gelatinolytic and caseinolytic activity compared to native bovine articular cartilage. Enzyme activity of the combined cultures was at a level intermediate between that of photo‐oxidized cartilage and native cartilage cultures alone. In contrast to normal cartilage, living chondrocytes were not visible in photo‐oxidized cartilage using live/dead staining. 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K.</creatorcontrib><creatorcontrib>VON RECHENBERG, B.</creatorcontrib><creatorcontrib>BITTMANN, P.</creatorcontrib><creatorcontrib>NADLER, D.</creatorcontrib><creatorcontrib>ZLINSZKY, K.</creatorcontrib><creatorcontrib>AUER, J. A.</creatorcontrib><title>In Vitro Studies of a Photo-oxidized Bovine Articular Cartilage</title><title>Journal of veterinary medicine. Series A</title><addtitle>J Vet Med A Physiol Pathol Clin Med</addtitle><description>Bovine articular cartilage was photo‐oxidized and cultured with native articular bovine cartilage and synovial membrane to study the interaction between these tissues mimicking the physiological situation in the joint. The photo‐oxidation was applied as a pretreatment of cartilage for future use in cartilage resurfacing procedures in joints. Properties of the transplant were assessed by testing the production of local mediators, such as nitric oxide (NO) and prostaglandin E2 (PGE2), and neutral metalloproteinase activities under normal conditions and after stimulation with various stimulants representative of inflammatory changes in pathophysiological conditions. Unlike normal cartilage photo‐oxidized cartilage did not release significant amounts of NO and PGE2 and showed less gelatinolytic and caseinolytic activity compared to native bovine articular cartilage. Enzyme activity of the combined cultures was at a level intermediate between that of photo‐oxidized cartilage and native cartilage cultures alone. In contrast to normal cartilage, living chondrocytes were not visible in photo‐oxidized cartilage using live/dead staining. These results indicate, that the photo‐oxidized cartilage may have a beneficial effect on adjacent native host cartilage and therefore be a suitable transplant for use in in vivo experiments.</description><subject>Animals</subject><subject>Cartilage, Articular - cytology</subject><subject>Cartilage, Articular - metabolism</subject><subject>Cartilage, Articular - radiation effects</subject><subject>Cattle</subject><subject>Cells, Cultured</subject><subject>Dinoprostone - biosynthesis</subject><subject>Humerus</subject><subject>Light</subject><subject>Metalloendopeptidases - biosynthesis</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Oxidation-Reduction</subject><subject>Shoulder Joint</subject><subject>Synovial Membrane - cytology</subject><subject>Synovial Membrane - metabolism</subject><subject>Synovial Membrane - radiation effects</subject><issn>0931-184X</issn><issn>1439-0442</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqNkMtOwzAQRS0EoqXwC8grdgl-JU4WCLXlKVVQifLYWU4yAZe0BjuBwteT0qpsmc2MNPfMSAchTElIiYiPpyEVPA2IECxkhLCQEJ7IcLGFupvFNuqSlNOAJuKpg_a8n5K2UiF3UYfSlPKERV10ej3HD6Z2Ft_VTWHAY1tijccvtraBXZjCfEOBB_bDzAH3XW3yptIOD3U7VvoZ9tFOqSsPB-veQ_cX55PhVTC6vbwe9kdBLgiXAcQiEZBLmRUs4gSk1pCVMbBU51EUZVJzKhLKOGcloSLTIo6LQghCtGjXJe-ho9XdN2ffG_C1mhmfQ1XpOdjGK0mjmLA0bYPJKpg7672DUr05M9PuS1GilvLUVC0dqaUjtZSnfuWpRYsern802QyKP3Btqw2crAKfpoKvfx9Wk8H5WTu1fLDija9hseG1e1Wx5DJSjzeXSo5jOWDJnRrzHx3nizQ</recordid><startdate>200202</startdate><enddate>200202</enddate><creator>AKENS, M. 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K.</creatorcontrib><creatorcontrib>VON RECHENBERG, B.</creatorcontrib><creatorcontrib>BITTMANN, P.</creatorcontrib><creatorcontrib>NADLER, D.</creatorcontrib><creatorcontrib>ZLINSZKY, K.</creatorcontrib><creatorcontrib>AUER, J. A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of veterinary medicine. Series A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>AKENS, M. K.</au><au>VON RECHENBERG, B.</au><au>BITTMANN, P.</au><au>NADLER, D.</au><au>ZLINSZKY, K.</au><au>AUER, J. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vitro Studies of a Photo-oxidized Bovine Articular Cartilage</atitle><jtitle>Journal of veterinary medicine. Series A</jtitle><addtitle>J Vet Med A Physiol Pathol Clin Med</addtitle><date>2002-02</date><risdate>2002</risdate><volume>49</volume><issue>1</issue><spage>39</spage><epage>45</epage><pages>39-45</pages><issn>0931-184X</issn><eissn>1439-0442</eissn><abstract>Bovine articular cartilage was photo‐oxidized and cultured with native articular bovine cartilage and synovial membrane to study the interaction between these tissues mimicking the physiological situation in the joint. The photo‐oxidation was applied as a pretreatment of cartilage for future use in cartilage resurfacing procedures in joints. 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These results indicate, that the photo‐oxidized cartilage may have a beneficial effect on adjacent native host cartilage and therefore be a suitable transplant for use in in vivo experiments.</abstract><cop>Berlin Germany</cop><pub>Blackwell Wissenschafts-Verlag</pub><pmid>11913825</pmid><doi>10.1046/j.1439-0442.2002.00387.x</doi><tpages>7</tpages></addata></record> |
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subjects | Animals Cartilage, Articular - cytology Cartilage, Articular - metabolism Cartilage, Articular - radiation effects Cattle Cells, Cultured Dinoprostone - biosynthesis Humerus Light Metalloendopeptidases - biosynthesis Nitric Oxide - biosynthesis Oxidation-Reduction Shoulder Joint Synovial Membrane - cytology Synovial Membrane - metabolism Synovial Membrane - radiation effects |
title | In Vitro Studies of a Photo-oxidized Bovine Articular Cartilage |
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