Collagen expression and biomechanical response to human recombinant transforming growth factor beta (rhTGF-β2) in the healing rabbit MCL

We investigated biomechanical and collagen expression in a healing bilateral rabbit medial collateral ligament (MCL) model to human recombinant transforming growth factor beta (rhTGF-β2) at three and six weeks. Each rabbit had rhTGF-β2 in a bioabsorbable pellet administered in one side, with the con...

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Published in:Journal of orthopaedic research 2002-03, Vol.20 (2), p.318-324
Main Authors: Spindler, K.P, Dawson, J.M, Stahlman, G.C, Davidson, J.M, Nanney, L.B
Format: Article
Language:English
Subjects:
Animals
Collagen Type I - biosynthesis
Collagen Type I - genetics
Disease Models, Animal
Elasticity - drug effects
Hindlimb - drug effects
Hindlimb - metabolism
Hindlimb - physiopathology
Humans
In Situ Hybridization
Knee Injuries - drug therapy
Knee Injuries - metabolism
Knee Injuries - physiopathology
Male
Medial Collateral Ligament, Knee - drug effects
Medial Collateral Ligament, Knee - metabolism
Medial Collateral Ligament, Knee - physiopathology
Rabbits
Range of Motion, Articular - drug effects
Range of Motion, Articular - physiology
Recombinant Proteins - therapeutic use
RNA, Messenger - metabolism
Transforming Growth Factor beta - therapeutic use
Transforming Growth Factor beta2
Weight-Bearing
Wound Healing - drug effects
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Dawson, J.M
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description We investigated biomechanical and collagen expression in a healing bilateral rabbit medial collateral ligament (MCL) model to human recombinant transforming growth factor beta (rhTGF-β2) at three and six weeks. Each rabbit had rhTGF-β2 in a bioabsorbable pellet administered in one side, with the contralateral side serving as control (no rhTGF-β2). All MCL healed with rhTGF-β2 producing a profoundly increased scar mass at three weeks which decreased in size toward control at six weeks. In-situ hybridization demonstrated collagen expression (type I and III) no different than control at three weeks, but by six weeks elevated expression of type I was seen. Biomechanical analysis at three weeks showed no effect of rhTGF-β2 on structural properties. However, at six weeks rhTGF-β2 significantly inhibited both the maximum load ( p
doi_str_mv 10.1016/S0736-0266(01)00107-3
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Orthop. Res</addtitle><description>We investigated biomechanical and collagen expression in a healing bilateral rabbit medial collateral ligament (MCL) model to human recombinant transforming growth factor beta (rhTGF-β2) at three and six weeks. Each rabbit had rhTGF-β2 in a bioabsorbable pellet administered in one side, with the contralateral side serving as control (no rhTGF-β2). All MCL healed with rhTGF-β2 producing a profoundly increased scar mass at three weeks which decreased in size toward control at six weeks. In-situ hybridization demonstrated collagen expression (type I and III) no different than control at three weeks, but by six weeks elevated expression of type I was seen. Biomechanical analysis at three weeks showed no effect of rhTGF-β2 on structural properties. However, at six weeks rhTGF-β2 significantly inhibited both the maximum load ( p&lt;0.05) and energy absorbed ( p&lt;0.05) with no change in stiffness. 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subjects Animals
Collagen Type I - biosynthesis
Collagen Type I - genetics
Disease Models, Animal
Elasticity - drug effects
Hindlimb - drug effects
Hindlimb - metabolism
Hindlimb - physiopathology
Humans
In Situ Hybridization
Knee Injuries - drug therapy
Knee Injuries - metabolism
Knee Injuries - physiopathology
Male
Medial Collateral Ligament, Knee - drug effects
Medial Collateral Ligament, Knee - metabolism
Medial Collateral Ligament, Knee - physiopathology
Rabbits
Range of Motion, Articular - drug effects
Range of Motion, Articular - physiology
Recombinant Proteins - therapeutic use
RNA, Messenger - metabolism
Transforming Growth Factor beta - therapeutic use
Transforming Growth Factor beta2
Weight-Bearing
Wound Healing - drug effects
title Collagen expression and biomechanical response to human recombinant transforming growth factor beta (rhTGF-β2) in the healing rabbit MCL
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