Cytoskeletal responses during early development of the downy mildew of grapevine (Plasmopara viticola)

• Published in: Protoplasma 2002-02, Vol.219 (1-2), p.13-22
• Main Authors: Riemann, M, Büche, C, Kassemeyer, H H, Nick, P
• Format: Article
• Language: English
• Subjects: Actins - metabolism; Bridged Bicyclo Compounds, Heterocyclic - pharmacology; Carbamates - pharmacology; Cell Polarity; Cytoplasmic Vesicles - metabolism; Cytoskeleton - drug effects; Cytoskeleton - metabolism; Oomycetes - cytology; Oomycetes - drug effects; Oomycetes - growth & development; Spores, Fungal - cytology; Spores, Fungal - drug effects; Spores, Fungal - growth & development; Thiazoles - pharmacology; Thiazolidines; Time Factors; Tubulin - metabolism; Vitis - microbiology
• ISSN: 0033-183X
• DOI: 10.1007/s007090200001

A host-free system was established to induce the early development of the obligate biotrophic pathogen Plasmopara viticola, the downy mildew of grapevine. This system was used to study cytoskeletal responses during encystation and germ tube formation. During these processes, both the actin and the tubulin cytoskeleton show a stage-specific pattern of distribution. Elimination of the cytoskeleton by the actin drug latrunculin B and the microtubule drug ethyl-N-phenyl-carbamate did not affect the release of mobile zoospores from the sporangia, nor the encystation process, but efficiently inhibited the formation of a germ tube. The data are discussed with respect to a role of both actin and microtubules for the establishment of the cell polarity guiding the emergence and the growth of the germ tube.