Erythrocyte zinc and carbonic anhydrase levels in nondialyzed chronic kidney disease patients

Objective: The aim of the present study was to determine the erythrocyte CAI and CAII concentrations in nondialyzed chronic kidney disease patients, and observe the relationship with acidosis, zinc, anemia, and iron supplementation. Methods: Erythrocyte CA concentrations were measured in nondialyzed...

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Bibliographic Details
Published in:Clinical biochemistry 2004, Vol.37 (1), p.67-71
Main Authors: Mafra, Denise, Cozzolino, Silvia M.F
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Anemia
Anemia - blood
Carbonic anhydrase
Carbonic Anhydrases - blood
Chronic Disease
Chronic kidney disease
ELISA
Enzyme-Linked Immunosorbent Assay - methods
Erythrocytes - chemistry
Erythrocytes - enzymology
Female
Humans
Iron
Iron - blood
Kidney Diseases - blood
Male
Middle Aged
Spectrophotometry, Atomic - methods
Zinc
Zinc - blood
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Summary:Objective: The aim of the present study was to determine the erythrocyte CAI and CAII concentrations in nondialyzed chronic kidney disease patients, and observe the relationship with acidosis, zinc, anemia, and iron supplementation. Methods: Erythrocyte CA concentrations were measured in nondialyzed patients ( n = 38) using enzyme-linked immunosorbent assays (ELISA). The zinc concentration was determined by atomic absorption spectrophotometer. Results and conclusions: The CA levels were observed to be increased in the patients. The mean erythrocyte zinc concentration was also high and the levels of zinc in plasma were baseline values. Correlation was found between CAI and erythrocytes Zn ( r = 0.46; P = 0.003), but there was no correlation with ferritin or pH and bicarbonate. The CA levels did not change after iron supplementation, but the zinc erythrocyte levels were reduced. It is concluded that the CA erythrocyte concentration in CKD patients is increased but this cannot be explained by iron deficiency or acidosis.
ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2003.03.001