Novel Ligands for the Investigation of Imidazoline Receptors and Their Binding Proteins

: New ligands for imidazoline receptors are described so that these receptors can be more fully explored and understood. BU224, (2‐(4,5‐dihydroimidaz‐2‐yl)‐quinoline, shows high affinity and is selective for the imidazoline‐2 (I2) class of receptors. BU224 was tested in the rat Porsolt forced swim p...

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Bibliographic Details
Published in:Annals of the New York Academy of Sciences 2003-12, Vol.1009 (1), p.302-308
Main Authors: HUDSON, AL, TYACKE, RJ, LALIES, MD, DAVIES, N, FINN, DP, MARTÍ, O, ROBINSON, E, HUSBANDS, S, MINCHIN, MCW, KIMURA, A, NUTT, DJ
Format: Article
Language:English
Subjects:
2BFI
Animals
Autoradiography
Behavior, Animal - physiology
Brain Chemistry
Brain Diseases - metabolism
BU224
creatine kinase
Humans
Imidazoles - chemistry
Imidazoles - metabolism
imidazoline binding sites
Imidazoline Receptors
Ligands
Mice
Molecular Structure
Rabbits
Radioligand Assay
Rats
Receptors, Adrenergic, alpha-2 - metabolism
Receptors, Drug - metabolism
Tomography, Emission-Computed
Tritium - metabolism
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Summary:: New ligands for imidazoline receptors are described so that these receptors can be more fully explored and understood. BU224, (2‐(4,5‐dihydroimidaz‐2‐yl)‐quinoline, shows high affinity and is selective for the imidazoline‐2 (I2) class of receptors. BU224 was tested in the rat Porsolt forced swim paradigm where it was found to decrease time spent immobile and increase the time spent swimming, consistent with an antidepressant profile. BU224 was tritiated and, in radioligand binding studies, was found to label a single population of saturable sites with high affinity. In vitro brain autoradiography with [3H]BU224 also showed a pattern of distribution similar to the known labeling of I2 receptors. A new series of four 2BFI (2‐(benzofuranyl)‐2‐imidazoline) derivatives were investigated as potential ligands for imaging brain I2 receptors using positron emission tomography (PET). At least two, BU20012 and BU20013, retained high affinity and moderate selectivity and penetrated the brain when administered peripherally in the mouse. 2BFI has undergone the Mannich reaction to immobilized diaminodipropyl amine to fabricate an affinity column, which was used to isolate a protein from rabbit brain; this protein was sequenced and identified as the enzyme creatine kinase.
ISSN:0077-8923
1749-6632
DOI:10.1196/annals.1304.039