Paternity testing after pregnancy termination using laser microdissection of chorionic villi

We report an unusual case of paternity testing from residues of chorionic villi 5 weeks after pregnancy termination. The autopsy of a 32-year-old female homicide victim revealed the presence of intact chorionic villi at the former placenta implantation site. Fetal cells were selectively isolated by...

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Bibliographic Details
Published in:International journal of legal medicine 2002-02, Vol.116 (1), p.39-42
Main Authors: Bauer, Martin, Thalheimer, Andreas, Patzelt, Dieter
Format: Article
Language:English
Subjects:
Abortion, Induced
Adult
Autopsy - methods
Chorionic Villi Sampling - methods
Female
Homicide
Humans
Lasers
Male
Paternity
Pregnancy
Tandem Repeat Sequences - genetics
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Summary:We report an unusual case of paternity testing from residues of chorionic villi 5 weeks after pregnancy termination. The autopsy of a 32-year-old female homicide victim revealed the presence of intact chorionic villi at the former placenta implantation site. Fetal cells were selectively isolated by laser-induced microdissection of the remaining villi to avoid contamination with maternal DNA. Simultaneous amplification of 12 STR loci in 2 PCR reactions resulted in a combined probability of paternity of 99.94%. This case demonstrates that laser-assisted microdissection and multiplex STR typing provide tools for paternity testing performed on endometrial mucosa long after the product of conception was removed by therapeutic abortion.
ISSN:0937-9827
1437-1596
DOI:10.1007/s004140000197