Drosophila CLOCK Protein Is under Posttranscriptional Control and Influences Light-Induced Activity

In the Drosophila circadian clock, daily cycles in the RNA levels of dclock (dClk) are antiphase to those of period ( per). We altered the timing/levels of dClk expression by generating transgenic flies whereby per circadian regulatory sequences were used to drive rhythmic transcription of dClk. The...

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Bibliographic Details
Published in:Neuron (Cambridge, Mass.) Mass.), 2002-03, Vol.34 (1), p.69-81
Main Authors: Kim, Eun Young, Bae, Kiho, Ng, Fanny S., Glossop, Nick R.J., Hardin, Paul E., Edery, Isaac
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified - genetics
Animals, Genetically Modified - metabolism
Circadian rhythm
Circadian Rhythm - physiology
CLOCK protein
CLOCK Proteins
dclock (dClk) gene
Drosophila melanogaster
Drosophila melanogaster - genetics
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Drosophila Proteins - physiology
Insect Proteins - genetics
Insect Proteins - metabolism
Insects
Kinases
Light
Male
Motor Activity - physiology
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Period Circadian Proteins
Phosphorylation
Proteins
RNA Processing, Post-Transcriptional - physiology
RNA, Messenger - genetics
RNA, Messenger - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
Transcription Factors - physiology
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Summary:In the Drosophila circadian clock, daily cycles in the RNA levels of dclock (dClk) are antiphase to those of period ( per). We altered the timing/levels of dClk expression by generating transgenic flies whereby per circadian regulatory sequences were used to drive rhythmic transcription of dClk. The results indicate that posttranscriptional mechanisms make substantial contributions to the temporal changes in the abundance of the dCLK protein. Circadian regulation is largely unaffected in the transgenic per- dClk flies despite higher mean levels of dCLK. However, in per-dClk flies the duration of morning activity is lengthened in light-dark cycles and light pulses evoke longer lasting bouts of activity. Our findings suggest that, in addition to a role in generating circadian rhythms, dCLK modulates the direct effects of light on locomotion.
ISSN:0896-6273
1097-4199
DOI:10.1016/S0896-6273(02)00639-6