Effect of Postmortem Delay on Imidazoline Receptor-Binding Proteins in Human and Mouse Brain

: Immunoreactive proteins of 45‐kD and 29/30‐kD doublet bands are candidate imidazoline receptor binding proteins (IRBP) based on associations with I1 or I2 binding sites, respectively. It was reported that the density of cortical membrane 29/30‐kD I2 protein is diminished whereas a 45‐kD I1 protein...

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Bibliographic Details
Published in:Annals of the New York Academy of Sciences 2003-12, Vol.1009 (1), p.341-346
Main Authors: MA, JOHN K., ZHU, HE, PILETZ, JOHN E.
Format: Article
Language:English
Subjects:
Adult
Animals
antiserum
Brain - metabolism
Depression - metabolism
Depression - pathology
Female
Humans
imidazoline receptor
Imidazoline Receptors
IRAS
Male
Mice
Mice, Inbred C57BL
Molecular Weight
Nerve Tissue Proteins - chemistry
Nerve Tissue Proteins - metabolism
postmortem
Protein Binding
Receptors, Drug - chemistry
Receptors, Drug - metabolism
Suicide
Time Factors
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Summary:: Immunoreactive proteins of 45‐kD and 29/30‐kD doublet bands are candidate imidazoline receptor binding proteins (IRBP) based on associations with I1 or I2 binding sites, respectively. It was reported that the density of cortical membrane 29/30‐kD I2 protein is diminished whereas a 45‐kD I1 protein is increased in depressed suicide victims versus controls. IRBP immunoreactive bands of similar size have been suggested to be breakdown products of the 170‐kD protein known as IRAS (putative full‐length I1 receptor). This study compares nonpathologic human brains collected and frozen after postmortem delays of 13.4 hours 6 1.7 (SEM) with brains of longer postmortem delays (26.1 hours 6 1.2). The fresher human brains possessed more full‐length IRAS (P5 0.05). In another study, the postmortem decay of IRBP bands in mouse brain was shown to be linear over time. The results are relevant to previous studies of IRBP bands in postmortem brains of depressed suicide victims.
ISSN:0077-8923
1749-6632
DOI:10.1196/annals.1304.043