IL-15 regulation in human endometrial stromal cells

A greater knowledge of IL-5 regulation within human endometrium is important in understanding key reproductive events and uterine Natural Killer cell function. In the present study, expression of IL-15 mRNA was shown to be up-regulated by both PGE(2) and IFN-gamma in cultures of human endometrial st...

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Bibliographic Details
Published in:The journal of clinical endocrinology and metabolism 2002-04, Vol.87 (4), p.1898-1901
Main Authors: DUNN, Carolyn L, CRITCHLEY, Hilary O. D, KELLY, Rodney W
Format: Article
Language:English
Subjects:
8-Bromo Cyclic Adenosine Monophosphate - pharmacology
Adult
Biological and medical sciences
Cells, Cultured
Dinoprostone - pharmacology
Drug Combinations
Endometrium - drug effects
Endometrium - metabolism
Female
Fundamental and applied biological sciences. Psychology
Hormone metabolism and regulation
Humans
Interferon-gamma - metabolism
Interferon-gamma - pharmacology
Interleukin-15 - genetics
Interleukin-15 - metabolism
Mammalian male genital system
Medroxyprogesterone Acetate - pharmacology
Menstrual Cycle - physiology
Pregnancy. Parturition. Lactation
Progesterone Congeners
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Stromal Cells - drug effects
Stromal Cells - metabolism
Up-Regulation
Vertebrates: reproduction
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Summary:A greater knowledge of IL-5 regulation within human endometrium is important in understanding key reproductive events and uterine Natural Killer cell function. In the present study, expression of IL-15 mRNA was shown to be up-regulated by both PGE(2) and IFN-gamma in cultures of human endometrial stromal cells (ESC). Release of IL-15 protein was also shown to be under the control of PGE(2) and IFN-gamma using an enzyme-linked immunosorbent assay for IL-15. In addition, 8-Bromo cAMP was able to increase IL-15 release from ESCs (P < 0.005) implying the actions of PGE(2) may be via this second messenger. Addition of a progestin appeared to enhance these effects. Real-time quantitative PCR has demonstrated an up-regulation in IL-15 mRNA expression in the late secretory phase of the menstrual cycle (P < 0.005) and a progressive rise in IFN-gamma expression throughout the secretory phase and into first trimester decidua. These results suggest that IL-15 regulation in the human endometrium is complex and that hormonal control may be indirect.
ISSN:0021-972X
1945-7197
DOI:10.1210/jc.87.4.1898