Deoxyribozyme-Based Logic Gates

We report herein a set of deoxyribozyme-based logic gates capable of generating any Boolean function. We construct basic NOT and AND gates, followed by the more complex XOR gate. These gates were constructed through a modular design that combines molecular beacon stem-loops with hammerhead-type deox...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2002-04, Vol.124 (14), p.3555-3561
Main Authors: Stojanovic, Milan N, Mitchell, Tiffany Elizabeth, Stefanovic, Darko
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Base Sequence
Biological and medical sciences
Biosensing Techniques
DNA, Catalytic - chemistry
Drug Design
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Logic
Molecular Sequence Data
Nucleic Acid Conformation
Nucleic acids
Oligonucleotides - chemistry
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Summary:We report herein a set of deoxyribozyme-based logic gates capable of generating any Boolean function. We construct basic NOT and AND gates, followed by the more complex XOR gate. These gates were constructed through a modular design that combines molecular beacon stem-loops with hammerhead-type deoxyribozymes. Importantly, as the gates have oligonucleotides as both inputs and output, they open the possibility of communication between various computation elements in solution. The operation of these gates is conveniently connected to a fluorescent readout.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja016756v