Thromboplastin-thrombomodulin-mediated time: a new global test sensitive to protein S deficiency and increased levels of factors II, V, VII and X

Unitat d'Hemostasia i Trombosi, Hospital de la Santa Creu i Sant Pau, Avgda San Antoni M Claret 167, 08025 Barcelona, Spain. mborrell@hsp.santpau.es BACKGROUND AND OBJECTIVES: A new test for screening the procoagulant capacity of plasma is described and evaluated. This test is based on the coag...

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Bibliographic Details
Published in:Haematologica (Roma) 2002-04, Vol.87 (4), p.415-419
Main Authors: Borrell, M, Llobet, D, Ortin, R, Felices, R, Vallve, C, Mateo, J, Souto, J, Fontcuberta, J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blood Coagulation - drug effects
Blood Coagulation Factors - pharmacology
Blood Coagulation Tests - methods
Blood Coagulation Tests - standards
Case-Control Studies
Female
Hematologic and hematopoietic diseases
Humans
Male
Medical sciences
Platelet diseases and coagulopathies
Protein S Deficiency - diagnosis
Sensitivity and Specificity
Thrombomodulin
Thrombophilia - blood
Thrombophilia - diagnosis
Thromboplastin - pharmacology
Thromboplastin - standards
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Summary:Unitat d'Hemostasia i Trombosi, Hospital de la Santa Creu i Sant Pau, Avgda San Antoni M Claret 167, 08025 Barcelona, Spain. mborrell@hsp.santpau.es BACKGROUND AND OBJECTIVES: A new test for screening the procoagulant capacity of plasma is described and evaluated. This test is based on the coagulation of plasma initiated by thromboplastin (Tp) in the presence of thrombomodulin (TM). In a previous paper we reported that this test had a significant phenotypic and genetic correlation with thrombosis susceptibility. The present report describes the characteristics of the test and its sensitivity to the concentration of some hemostasis factors. DESIGN AND METHODS: Plasma from normal subjects, from individuals with various disorders of hemostasis and plasma with different concentrations of factors II, V, VII, VIII, X, fibrinogen, protein C and protein S were studied. The thromboplastin-thrombomodulin-mediated time (Tp-TMT) is measured after mixing 100 mL of plasma diluted 1/10 at 37 C with 100 mL of a solution composed of 2 parts of thromboplastin, 1 part of thrombomodulin at 30 U/mL and 1 part of Owren's buffer. The results are expressed as the ratio of the patient's clotting time to that of the control. Values were compared with Student's t test and the Mann-Whitney test. Differences were considered statistically significant when p
ISSN:0390-6078
1592-8721