Mild traumatic brain injury induces apoptotic cell death in the cortex that is preceded by decreases in cellular Bcl-2 immunoreactivity

Although mild traumatic brain injury is associated with behavioral dysfunction and histopathological alterations, few studies have assessed the temporal pattern of regional apoptosis following mild brain injury. Anesthetized rats were subjected to mild lateral fluid-percussion brain injury (1.1–1.3...

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Bibliographic Details
Published in:Neuroscience 2002-01, Vol.110 (4), p.605-616
Main Authors: Raghupathi, R, Conti, A.C, Graham, D.I, Krajewski, S, Reed, J.C, Grady, M.S, Trojanowski, J.Q, McIntosh, T.K
Format: Article
Language:English
Subjects:
Animals
apoptosis
Apoptosis - physiology
Bax
Bcl-2
bcl-2-Associated X Protein
Biological and medical sciences
Brain Injuries - metabolism
Brain Injuries - pathology
Brain Injuries - physiopathology
Cell Count
Cell Size - physiology
Cerebral Cortex - injuries
Cerebral Cortex - metabolism
Cerebral Cortex - pathology
DNA Fragmentation - physiology
Down-Regulation - physiology
head injury
hippocampus
Hippocampus - injuries
Hippocampus - metabolism
Hippocampus - pathology
Immunohistochemistry
In Situ Nick-End Labeling
Injuries of the nervous system and the skull. Diseases due to physical agents
Male
Medical sciences
Nerve Degeneration - pathology
Neurons - metabolism
Neurons - pathology
Proto-Oncogene Proteins - metabolism
Proto-Oncogene Proteins c-bcl-2 - metabolism
Rats
Rats, Sprague-Dawley
Traumas. Diseases due to physical agents
white matter
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Summary:Although mild traumatic brain injury is associated with behavioral dysfunction and histopathological alterations, few studies have assessed the temporal pattern of regional apoptosis following mild brain injury. Anesthetized rats were subjected to mild lateral fluid-percussion brain injury (1.1–1.3 atm), and brains were evaluated for the presence of in situ DNA fragmentation (terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling, TUNEL) and morphologic characteristics of apoptotic cell death (nuclear and cytoplasmic condensation, presence of apoptotic bodies). Significant numbers of apoptotic TUNEL(+) cells were observed in the injured parietal cortex and underlying white matter up to 72 h post-injury ( P
ISSN:0306-4522
1873-7544
DOI:10.1016/S0306-4522(01)00461-4